Ochaikul, Duangjail

Preferred name

Ochaikul, Duangjail

Alternative Name

Ochaikul, D.

Ochaikul, Duangjai

Ochaikul, Daungjai

daungjai.oc@kmitl.ac.th

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Description of Komagataeibacter gen. nov., with proposals of new combinations (Acetobacteraceae)
(2012-11-23)
Yamada, Yuzo
;
Yukphan, Pattaraporn
;
Vu, Huong Thi Lan
;
Muramatsu, Yuki
;
Ochaikul, Duangjai
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Production and quality improvement of the tropical fruit tamarind (Tamarindus indica Linn.) wine
(2018-05-01)
Pongkan, S.
;
Tilarux, P.
;
Charoensuk, K.
;
Ochaikul, D.
;
Suwanposri, A.
Tamarind (Tamarindus indica Linn.) is one of the most popular tropical fruit with many benefits in Thailand. It have been used as a food ingredient for a long time and has many therapeutic properties. Therefore, The research finding was to produce and improve the quality of tamarind wine. The two varieties of Saccharomyces cerevisiae var. burgundy and montachae were chosen for tamarind wine fermentation. Both varieties produced similar percentages of alcohol by volume but for the sensory evaluation showed that montachae was better than burgundy with the 3.87 ± 0.10 points. The optimal conditions for production of tamarind wine was 10% inoculum concentration, 5% tamarind juice and 20 °Brix total soluble solid with the 0.67 percentage of alcohol by volume and 3.63 ± 0.10 points from sensory evaluation. Then the quality of tamarind wine was improved by mixing tamarind juice with pineapple juice and roselle juice. The result showed that, production of wine using only tamarind juice was obtained the maximum sensory evaluation result with 3.58 ± 0.24 points. In conclusion, tamarind can be used as substrate for production of wine. It will be meet an alternative way to add value to tamarind. Moreover, the produced tamarind wine had a good taste which the acceptance from the consumers without the quality improvement.
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Statistical optimisation of culture conditions for biocellulose production by Komagataeibacter sp. PAP1 using soya bean whey
(2014-02-18)
Suwanposri, Amornrat
;
Yukphan, Pattaraporn
;
Yamada, Yuzo
;
Ochaikul, Duangjai
A low-cost medium based on soya bean whey (SBW) was prepared as a by-product from agriculture and optimised for biocellulose (BC) production by Komagataeibacter sp. PAP1. The optimal conditions for BC production are: pH 6.21, 1.61% ethanol concentration (v/v) and 28.4°C. The use of optimised medium based on SBW increased BC production 3.6 fold compared to standard Hestrin-Schramm (HS) medium. The BC film produced from the optimised medium was stronger and more impermeable to water vapour and oxygen compared to the one produced from the standard HS medium. These properties allow SBW film to be developed into eco-friendly food packaging for oxygen-sensitive products. Its high water absorption capacity allows its use in biomedical application such as wound dressing. Our results demonstrate that SBW can be used as an alternative low-cost substrate for BC production on commercial scale. © 2014 by Maejo University, San Sai, Chiang Mai, 50290 Thailand.
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Bioethanol fermentation from acid/base-treated water hyacinth biomass using fermentation yeasts saccharomyces cerevisiae yrk 017 and candida shehatae ATCC 22984
(2019-01-01)
Ochaikul, Duangjai
;
Jongmeesuk, Atcharaporn
;
Maneeruttanarungroj, Cherdsak
Water hyacinth is considered as one of most abundant lignocellulosic feedstock in Thailand. It has been selected as a substrate for producing ethanol at an economically feasible manner. This study focuses on the bioethanol production from enzyme-treated hydrolysate of acid/base pretreatment water hyacinth. Two different pretreatment methods of water hyacinth were used including either 2 %(v/v) sulfuric acid or 2 %(w/v) sodium hydroxide at 121°C for 15 min. After acid/base pretreatments, the biomass residues in order to release glucose molecules were treated with a commercial cellulase enzyme complex (ACCELLERASE® 1500) at a concentration of 0.30 mL enzyme/g biomass residue. Two yeast species consisting of Saccharomyces cerevisiae YRK 017 and Candida shehatae ATCC 22984 were separately used as inoculum in fermentation process of the hydrolysate from enzymatic treatments. The results showed that ethanol yield obtained from acid pretreatment was slightly higher than the yield from base pretreatment. In acid pretreatment, S. cerevisiae YRK 017 could produce bioethanol in a yield of 3.82 ± 0.10 g/L, whereas C. shehatae ATCC 22984 could produce yielding 2.85 ± 0.04 g/L.
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Evaluation of antioxidant capacities of green microalgae
(2014-02-01)
Choochote, Weena
;
Suklampoo, Linchong
;
Ochaikul, Duangjai
Three strains of green microalgae, Chlorococcum sp.C53, Chlorella sp. E53, and Chlorella sp.ED53 were studied for their antioxidant activities. Crude extracts of these microalgae in hot water and in ethanol were examined for their total phenolic contents and for their antioxidant capacities. In order to determine their phenolic contents, the Folin-Ciocalteu method was used. As for the determination of their antioxidant capacities, four different assays were used: (1) total antioxidant capacity determination; (2) DPPH radical scavenging assay; (3) ferrous ion chelating ability assay; and (4) inhibition of lipid peroxidation (using thiobarbituric acid reactive substance). For all the strains we have studied, their ethanolic extract showed more antioxidant activities than their hot water extract. Categorically, the ethanolic extract of Chlorella sp.E53 exhibited both the highest total phenolic content of 35.5 ± 0.14 mg gallic acid equivalent (GAE) g-1 dry weight and the highest DPPH radical scavenging of 68.18 ± 0.38 % at 1.4 mg mL-1 (IC<inf>50</inf> 0.81 mg mL-1), whereas Chlorella sp.ED53 showed both the highest ferrous ion chelation activity of 42.78 ± 1.48 % at 1 mg mL-1 (IC<inf>50</inf> 1.23 mg mL-1) and the highest inhibition of lipid peroxidation of 87.96 ± 0.59 % at 4 mg mL-1. This high level of inhibition is comparable to 94.42 ± 1.39 % of butylated hydroxytoluene, a commercial synthetic antioxidant, at the same concentration. © 2013 Springer Science+Business Media Dordrecht.
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Subdivision of the genus Gluconacetobacter Yamada, Hoshino and Ishikawa 1998: The proposal of Komagatabacter gen. nov., for strains accommodated to the Gluconacetobacter xylinus group in the α-Proteobacteria
(2012-06-01)
Yamada, Yuzo
;
Yukphan, Pattaraporn
;
Vu, Huong Thi Lan
;
Muramatsu, Yuki
;
Ochaikul, Duangjai
The genus Gluconacetobacter is divided into two groups phylogenetically, phenotypically and ecologically: the Gluconacetobacter liquefaciens group and the Gluconacetobacter xylinus group. For the latter group, the genus Komagatabacter is newly introduced, and the type species of the new genus is designated as Komagatabacter xylinus (Brown 1886) comb. nov. Twelve species of the Gluconacetobacter xylinus group are transferred to the new genus as new combinations. © Springer-Verlag and the University of Milan 2011.
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Identification and biocellulose production of Gluconacetobacter strains isolated from tropical fruits in thailand
(2013-03-28)
Suwanposri, Amornrat
;
Yukphan, Pattaraporn
;
Yamada, Yuzo
;
Ochaikul, Daungjai
Two hundred and four strains of biocellulose (BC)-producing Gluconacetobacter strains were isolated from 48 rotten tropical fruits collected in Thailand. Twenty-nine representative isolates were selected from each of the 16 isolation sources and identified by morphological, physiological and biochemical characteristics and 16S rRNA gene sequence analysis. The selected 29 isolates were divided into seven subgroups within the Gluconacetobacter xylinus group of the genus Gluconacetobacter and identified as Gluconacetobacter oboediens (subgroup I, five isolates), Gluconacetobacter rhaeticus (subgroup II, one isolate), Gluconacetobacter hansenii (subgroup III, seven isolates), Gluconacetobacter swingsii (subgroup IV, two isolates) and Gluconacetobacter sucrofermentans (subgroup V, two isolates). The remaining isolates were grouped into subgroups VIa (three isolates) and VIb (nine isolates). All the isolates were cultured in Hestrin-Schramm (HS) medium statically at 30°C for 7 days to determine cellulose production capability. Of the 29 isolates, isolate PAP1 (subgroup VIb, unidentified) gave the highest yield (1.15 g/L) of BC. However, the BC yield increased threefold (3.5 g/L) when D-glucose in HS medium was replaced by D-mannitol. © 2013 by Maejo University, San Sai, Chiang Mai, 50290 Thailand. Reproduction is permitted for noncommercial purposes.

Ochaikul, Duangjail

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