Ochaikul, Duangjail
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Ochaikul, Duangjail
Alternative Name
Ochaikul, D.
Ochaikul, Duangjai
Ochaikul, Daungjai
Email
daungjai.oc@kmitl.ac.th
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Item type:Publication, Bioethanol production from cassava starch using co-culture of saccharolytic molds with Saccharomyces cerevisiae TISTR 5088(2024-08-01) ;Pimpisai, Timakorn ;Maneerattanarungroj, Cherdsak; Cassava serves as a cornerstone for sustainable bioethanol production in Thailand. The cassava root is highly valuable, comprising 50–70% starch on a dry weight basis. Starch can be converted into fermentable sugars through chemical or biological methods. This study focused on harnessing the enzymatic potential of saccharolytic molds, specifically Aspergillus oryzae TISTR 3086 or Amylomyces rouxii TISTR 3182, to facilitate saccharification of cassava starch, followed by fermentation using Saccharomyces cerevisiae TISTR 5088 for bioethanol production. Among the various configurations, the co-culture of Amy. rouxii TISTR 3182 with S. cerevisiae TISTR 5088 was the most effective, resulting in the highest ethanol yield. This finding was observed for both the separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) processes. Impressively, the SSF approach to ethanol productivity demonstrated superior results with an ethanol concentration of 25.4±0.3 g/l and an ethanol productivity of 0.53 g/l/h (yield of 1.40 g/g reducing sugars), surpassing the SHF approach (25.4±0.7 g/l, an ethanol productivity of 0.26 g/l/h, yield of 0.57 g/g reducing sugars) while also reducing the fermentation period. Further investigations into optimizing the conditions for ethanol production were carried out using a co-culture of Amy. rouxii TISTR 3182 with S. cerevisiae TISTR 5088 during SSF. This exploration revealed that employing 100 g/l cassava starch and initiating fermentation with a medium pH of 6.0 led to the highest ethanol concentration at 48 h. This process showed potential for ethanol production, harnessing the synergistic action of saccharolytic molds alongside yeast. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Production and quality improvement of the tropical fruit tamarind (Tamarindus indica Linn.) wine(2018-05-01) ;Pongkan, S. ;Tilarux, P. ;Charoensuk, K.; Suwanposri, A.Tamarind (Tamarindus indica Linn.) is one of the most popular tropical fruit with many benefits in Thailand. It have been used as a food ingredient for a long time and has many therapeutic properties. Therefore, The research finding was to produce and improve the quality of tamarind wine. The two varieties of Saccharomyces cerevisiae var. burgundy and montachae were chosen for tamarind wine fermentation. Both varieties produced similar percentages of alcohol by volume but for the sensory evaluation showed that montachae was better than burgundy with the 3.87 ± 0.10 points. The optimal conditions for production of tamarind wine was 10% inoculum concentration, 5% tamarind juice and 20 °Brix total soluble solid with the 0.67 percentage of alcohol by volume and 3.63 ± 0.10 points from sensory evaluation. Then the quality of tamarind wine was improved by mixing tamarind juice with pineapple juice and roselle juice. The result showed that, production of wine using only tamarind juice was obtained the maximum sensory evaluation result with 3.58 ± 0.24 points. In conclusion, tamarind can be used as substrate for production of wine. It will be meet an alternative way to add value to tamarind. Moreover, the produced tamarind wine had a good taste which the acceptance from the consumers without the quality improvement. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Bioethanol fermentation from acid/base-treated water hyacinth biomass using fermentation yeasts saccharomyces cerevisiae yrk 017 and candida shehatae ATCC 22984(2019-01-01); ;Jongmeesuk, AtcharapornWater hyacinth is considered as one of most abundant lignocellulosic feedstock in Thailand. It has been selected as a substrate for producing ethanol at an economically feasible manner. This study focuses on the bioethanol production from enzyme-treated hydrolysate of acid/base pretreatment water hyacinth. Two different pretreatment methods of water hyacinth were used including either 2 %(v/v) sulfuric acid or 2 %(w/v) sodium hydroxide at 121°C for 15 min. After acid/base pretreatments, the biomass residues in order to release glucose molecules were treated with a commercial cellulase enzyme complex (ACCELLERASE® 1500) at a concentration of 0.30 mL enzyme/g biomass residue. Two yeast species consisting of Saccharomyces cerevisiae YRK 017 and Candida shehatae ATCC 22984 were separately used as inoculum in fermentation process of the hydrolysate from enzymatic treatments. The results showed that ethanol yield obtained from acid pretreatment was slightly higher than the yield from base pretreatment. In acid pretreatment, S. cerevisiae YRK 017 could produce bioethanol in a yield of 3.82 ± 0.10 g/L, whereas C. shehatae ATCC 22984 could produce yielding 2.85 ± 0.04 g/L.
