Duangupama, Thitikorn

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Duangupama, Thitikorn
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thitikorn.du@kmitl.ac.th

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    Item type:Publication,
    Micromonospora solifontis sp. nov., an actinobacterium isolated from hot spring soil
    (2023-05-01)
    Duangupama, Thitikorn
    ;
    Pansomsuay, Rawirat
    ;
    Pittayakhajonwut, Pattama
    ;
    Intaraudom, Chakapong
    ;
    Suriyachadkun, Chanwit
    An actinobacterium strain, PPF5-17<sup>T</sup>, was isolated from hot spring soil collected from Chiang Rai province, Thailand. The strain exhibited morphological and chemotaxonomic properties similar to those of members of the genus Micromonospora. Colonies of PPF5-17<sup>T</sup> were strong pinkish red and turned black after sporulation in ISP 2 agar medium. Cells formed single spores directly on the substrate mycelium. Growth was observed from 15 to 45 °C and at pH 5–8. Maximum NaCl concentration for growth was 3% (w/v). PPF5-17<sup>T</sup> was found to have meso-diaminopimelic acid, xylose, mannose and glucose in the whole-cell hydrolysate. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannosides were observed as the membrane phospholipids. MK-10(H<inf>6</inf> ), MK-9(H<inf>6</inf> ), MK-10(H<inf>4</inf> ) and MK-9(H<inf>4</inf> ) were the major menaquinones. The predominant cellular fatty acids were iso-C<inf>15:0</inf>, iso-C<inf>17:0</inf>, anteiso-C<inf>17:0</inf> and iso-C<inf>16:0</inf> . PPF5-17<sup>T</sup> shared the highest 16S rRNA gene sequence similarity with Micromonospora fluminis LMG 30467<sup>T</sup> (99.3%). A genome-based taxonomic study revealed that PPF5-17<sup>T</sup> was closely related to Micromonospora aurantinigra DSM 44815<sup>T</sup> in the phylogenomic tree with an average nucleotide identity by blast (ANIb) of 87.7% and a digital DNA–DNA hybridization (dDDH) value of, 36.1% which were below the threshold values for delineation of a novel species. Moreover, PPF5-17<sup>T</sup> could be distinguished from its closest neighbours, M. fluminis LMG 30467<sup>T</sup> and M. aurantinigra DSM 44815<sup>T</sup>, with respect to a broad range of phenotypic properties. Thus, PPF5-17<sup>T</sup> represents a novel species, for which the name Micromonospora solifontis sp. nov. is proposed. The type strain is PPF5-17<sup>T</sup> (= TBRC 8478<sup>T</sup> = NBRC 113441<sup>T</sup>).
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    Item type:Publication,
    Micromonospora thermarum sp. nov., an actinobacterium isolated from hot spring soil
    (2023-04-01)
    Pansomsuay, Rawirat
    ;
    Duangupama, Thitikorn
    ;
    Pittayakhajonwut, Pattama
    ;
    Intaraudom, Chakapong
    ;
    Suriyachadkun, Chanwit
    An actinomycete, designated strain HSS6-12<sup>T</sup>, was isolated from hot spring sediment collected from Ranong province, Thailand. The strain showed taxonomic characteristics consistent with those of members of the genus Micromonospora. HSS6-12<sup>T</sup> produced a single spore directly on the substrate mycelium, and no aerial mycelium was detected. The isomer of diamino acid presented in cell wall peptidoglycan was meso-diaminopimelic acid. Arabinose, xylose, glucose, and ribose were detected in whole-cell hydrolysates. MK-10(H<inf>4</inf>), MK-9(H<inf>4</inf>), and MK-10(H<inf>6</inf>) were major menaquinones. Major cellular fatty acids were iso-C<inf>16:0</inf>, iso-C<inf>15:0</inf>, and iso-C<inf>17:0</inf>. Phospholipid profile was composed of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, and phosphatidylinositolmannosides. 16S rRNA gene analysis revealed that HSS6-12<sup>T</sup> shared the highest 16S rRNA gene sequence similarity with Micromonospora inositola DSM 43819<sup>T</sup> (99.3%). In contrast, the genome analysis showed that HSS6-12<sup>T</sup> formed a tight taxonomic position in a phylogenomic tree with Micromonospora endolithica DSM 44398<sup>T</sup>. Moreover, the average nucleotide identity-blast, the digital DNA-DNA hybridization, and the average amino acid identity values between HSS6-12<sup>T</sup> and M. inositola DSM 43819<sup>T</sup> and M. endolithica DSM 44398<sup>T</sup> were 83.1–84.0%, 27.5–28.7%, and 80.4–82.2%, respectively, indicating that HSS6-12<sup>T</sup> was different species with both closely related Micromonospora-type strains. In addition, HSS6-12<sup>T</sup> could be discriminated from its closely related type strains by many physiological and biochemical characteristics. Thus, HSS6-12<sup>T</sup> could be considered a novel species of the genus Micromonospora, and the name Micromonospora thermarum is proposed for the strain. The type strain is HSS6-12<sup>T</sup> (= BCC 41915<sup>T</sup> = JCM 17127<sup>T</sup>).