Duangupama, Thitikorn

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Duangupama, Thitikorn
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thitikorn.du@kmitl.ac.th

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    Item type:Publication,
    Thermocatellispora soli sp. nov., isolated from hot spring soil
    (2019-01-01)
    Duangupama, Thitikorn
    ;
    Pittayakhajonwut, Pattama
    ;
    Thawai, Chitti
    An aerobic, spore-forming, actinomycete, designated strain CHM3-46 <sup>T</sup> , was isolated from soil in a hot spring pond located in Chiangmai province, Thailand. The strain exhibited taxonomic characteristics consistent with the genus Thermocatellispora. Strain CHM3-46 <sup>T</sup> produced short, straight chains of warty spores on aerial mycelia. The presence of meso-diaminopimelic acid was observed in the cell-wall peptidoglycan. The whole-cell reducing sugars were glucose, mannose, galacose and ribose. The phospholipids comprised phosphatidylmethylethanolamine, hydroxyphosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, four phosphoglycolipids and three unidentified phospholipids. The predominant menaquinones were MK-9(H <inf>4</inf> ), MK-9(H <inf>6</inf> ) and MK-9 (H <inf>8</inf> ). 10-methyl C <inf>17 : 0</inf> , C <inf>16 : 0</inf> , C <inf>17 : 0</inf> and iso-C <inf>16 : 0</inf> were identified as the main cellular fatty acids. The G+C content of the genomic DNA was 73.2 mol%. Analysis of the 16S rRNA gene sequence showed that strain CHM3-46T belonged to the genus Thermocatellispora, exhibiting the highest similarity to Thermocatellispora tengchongensis YIM 77521 <sup>T</sup> (98.5 %). Furthermore, a low DNA relatedness value (23.4 %±0.8) and several physiological and biochemical characteristic differences were detected between strain CHM3-46 <sup>T</sup> and its closest relative. Based on the taxonomic data, strain CHM3-46 <sup>T</sup> could be readily distinguished from its closest phylogenetic relative and represents a novel species, for which the name Thermocatellispora soli sp. nov. is proposed. The type strain is CHM3-46 <sup>T</sup> (=TBRC 7649 <sup>T</sup> =NBRC 113148 <sup>T</sup> ).