Houngkamhang, Nongluck

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Houngkamhang, Nongluck
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Email
nongluck.ho@kmitl.ac.th

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    Conjugation of antibody on gold nanoparticles for biosensors application
    (2020-01-01)
    Ta-Aithuak, Sarocha
    ;
    Loedsapchinda, Naruenard
    ;
    Houngkamhang, Nongluck
    Regarding to the unique optical property and biocompatible, gold nanoparticles have been widely used to functionalize with antibodies probe for testing specificity with their antigen targets. The antibody immobilized onto gold nanoparticles which synthesized by different methods were studied. Gold nanoparticles synthesized by citrate reduction method and by using poly(ethylene)glycol (PEG) coated gold nanoparticles were immobilized with IgM antibody by physical adsorption. Gold nanoparticles before and after functionalize with antibodies were characterized with fourier-transform infrared spectroscopy (FTIR) for functional group and UV-Vis spectroscopy for absorption wavelength. Finally, a specificity test was conducted using spot of anti-IgM antibody onto nitrocellulose membrane to confirm the bioactivity of antibodies attached to gold nanoparticles.
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    Gold nanorod-optical fiber for sensing biomolecular interaction
    (2020-01-01)
    Chueaiarrom, Channarong
    ;
    Loedsapchinda, Naruenard
    ;
    Puttharugsa, Chokchai
    ;
    Pipatpanukul, Chinnawut
    ;
    Srikhirin, Toemsak
    The optical fiber deposits with gold nanorods was successfully accomplished as a biosensor device which sensing to the refractive index change on the surrounding surface. The principle is based on the localized surface plasmon resonance (LSPR) occurs on the metal surface of gold nanorods (AuNRs). The gold nanorods were coated on the glass core fiber surface through the covalent bond. The uncladded area fiber optic was incubated in 5% of (3-Mercaptopropyl) trimethoxysilane (MPTMS) in ethanol for overnight to alter the thiol group on the surface. The gold nanorods with aspect ratio ⁓2.035 was deposited on thiol surface by incubation the optical fiber in gold nanorod solution for 6 hr. The sensor provides the sensitivity of 4.94×104 a.u./RIU. Moreover, antibody for blood grouping was immobilized on the AuNRs-optical fiber to detect the red blood cells. The AuNRs-optical fiber can detect A antigen on the immobilized anti-A surface with Δ%T of 0.98 and can detect B-antigen on the immobilized anti-B surface with Δ%T of 1.78. This work possesses possibility of a simple AuNRs-optical fiber to detect the biomolecular interaction. The authors would like to thank Thailand Center of Excellence in Physics for financial support.
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    Study of anti-a antibody conjugated gold nanoparticles as a biomolecular detection probe for a substance in saliva
    (2020-01-01)
    Loedsapchinda, Naruenard
    ;
    Ta-Aithuak, Sarocha
    ;
    Chueaiarrom, Channarong
    ;
    Srikhirin, Toemsak
    ;
    Houngkamhang, Nongluck
    In this research studied on the conjugation of antibodies on gold nanoparticles (GNPs) and the optimization in term of antibody concentration and albumin blocking concentration. Gold nanoparticles with diameter size of ~20 nm were synthesized via chemical reduction of HAuCl4 with trisodium citrate as reducing and stabilizing agent. Gold nanoparticles and anti-A antibody at various concentrations were mixed and nonspecific binding sites were blocked by using bovine serum albumin (BSA) at various concentrations. The antibody conjugated gold nanoparticles were tested by coagulation with 1% sodium chloride salt solution to obtain the optimal antibody concentration. BSA 50, 100 and 200 μg/mL were added in solution after antibody covered on gold nanoparticles. The solutions were characterized by measuring absorbance spectra. From the results found that the optimal concentration of antibodies and BSA blocking were 45 μg/mL and 50 μg/mL, respectively. The anti-A conjugated gold nanoparticles were tested with saliva of blood group A sample to monitor the specific interaction between antibody on gold surface and antigen in saliva via the change of absorbance spectra.