Houngkamhang, Nongluck

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Houngkamhang, Nongluck
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Email
nongluck.ho@kmitl.ac.th

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    Synthesis of Zeolite Y from Kaolin via hydrothermal method
    (2019-01-01)
    Krongkrachang, Pimpetch
    ;
    Thungngern, Pimpreeya
    ;
    Asawaworarit, Panuruj
    ;
    Houngkamhang, Nongluck
    ;
    Eiad-Ua, Apiluck
    ZeoliteY has been successfully synthesized via a two-step hydrothermal method with kaolin from Lampang province as a precursor. For the first hydrothermal, kaolin with an addition of sodium silicate (NaSi<inf>3</inf>O<inf>7</inf>) or tetraethyl orthosilicate (TEOS) was mixed with sodium hydroxide (NaOH) and autoclaved for 200<sup>o</sup>C at different reaction times for 6, 8 and 10 h, respectively. Sodium hydroxide and hydrochloric acid were added into the compound mixture to adjust its pH before it was formed into gel. After a second time hydrothermal at 90°C for 2 days, the solid product was filtered out, washed by deionized water and dried overnight. The zeolite was then characterized by X-ray diffraction spectroscopy (XRD) to identify type and crystallization, scanning electron microscopy (SEM) for morphology, Fourier transform infrared spectroscopy (FTIR) to identify its functional group and N<inf>2</inf> adsorption to study the adsorption/desorption isotherm and pore distribution. From N<inf>2</inf> adsorption-desorption isotherm, it is revealed that the zeolite obtained by this method have 571 m<sup>2</sup>/g surface area and pore size distribution around 4.89 nm.
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    Zeolite P from kaolin via hydrothermal method
    (2018-09-05)
    Eiad-Ua, Apiluck
    ;
    Amnaphiang, Phanwasa
    ;
    Asawaworarit, Panuruj
    ;
    Houngkamhang, Nongluck
    ;
    Chollacoop, Nuwong
    Zeolite P has been successfully synthesized from natural kaolin via two step hydrothermal process. The natural kaolin from Lampang, Thailand was studied for this research. In first hydrothermal, kaolin was mixed with sodium hydroxide solution at 200 <sup>o</sup>C for different reaction times from 3 - 5 hours, respectively.Sodium hydroxide and hydrochloric acid were added into the mixture to adjust their pH before they were formed into gel. Second hydrothermal process, the gel was kept of 90 <sup>o</sup>C for 3 days to obtain zeolite P. Synthesized zeolite P was characterized by X-ray diffraction (XRD) for identification the type and crystallization. Besides, the morphology was characterized by scanning electron microscopy)SEM.(The functional group was characterized by Fourier-transform infrared spectroscopy (FTIR). From this study, it was found that the increasing of reaction time in first hydrothermal activation and hydrochloric acid concentration lead to the high quality of zeolite P.
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    Gold-nanoparticle-based fiber optic sensor for sensing the refractive index of environmental solutions
    (2018-08-01)
    Houngkamhang, Nongluck
    ;
    Charoensuwan, Sittan
    ;
    Sonthipakdee, Onanong
    ;
    Nawattanapaiboon, Kawin
    ;
    Somboonkaew, Armote
    Here an optical fiber modified with gold nanoparticles was successfully fabricated to sense the refractive index of a chemical solution surrounding its surface, with the potential of utilizing this sensor as a biosensing device. Gold nanoparticles, with an average diameter ~20 nm, were synthesized via the citrate reduction method and used to functionalize the glass core of an optical fiber. The sensing principle, which is based on localized surface plasmon resonance, requires the metal gold nanoparticles to be exposed to both the incident and absorbing lights, with the potential detection capabilities determined from the observed light intensity measurement. The optical fiber was uncladded at its center to expose the glass core fiber, and gold nanoparticles were immobilized on the exposed surface using a silane coupling agent. The sensitivity of the gold-nanoparticle-modified optical fibers in measuring the refractive index changes of a solution was compared for unclad lengths ranging between 1 and 2 cm. The attenuation of light depended on both the refractive index of solution and the length of the unclad fiber. The results showed that the fiber sensitivity increased as the unclad length increased due to greater amount of gold on the surface. Antibody-A, which has a specific binding to antigen-A, was then covalently linked to the fiber surface via an amide bond to demonstrate the potential bio-sensing platform of this sensor. The antibody-A functionalized optical fiber was used to detect the red blood cell samples in groups A, B, and O, where it effectively detected both the specific and non-specific binding signals. This fiber optic biosensor therefore provides a low-cost and simple fabrication setup that has potential field applications. Moreover, this setup could potentially be applied to detect other types of whole cell samples.
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    Extended-gate field effect transistor (EGFET) for carbaryl pesticide detection based on enzyme inhibition assay
    (2017-01-01)
    Sasipongpana, Supanat
    ;
    Rayanasukha, Yossawat
    ;
    Prichanont, Seeroong
    ;
    Thanachayanont, Chanchana
    ;
    Porntheeraphat, Supanit
    An extended - gate filed effect transistor (EGFET) is a device which can be applied to chemical sensing devices. In this study, EGFET was constructed by using an indium tin oxide (ITO) glass substrate. The small piece ITO with sensing area around 3x3 mm<sup>2</sup> was used as carbaryl pesticide sensor based on enzyme inhibition assay. The prepared ITO-EGFET sensors were test with pH solutions to obtain sensitivity compared with commercial ion sensitive field effect transistor (ISFET). Preparation of ITO substrate for enzyme inhibition assay by dropped acetylcolinesterase enzyme at 0.5 Unit which were trapped in an agarose gel matrix onto substrates. This prepared ITO acts as extended gate sensing membrane for EGFET. The amounts of drop volume of enzyme were varied to obtain the highest signal for reaction with acetylcholine substrate. Cabaryl can inhibit enzyme function resulting in the decreasing signal of interaction between enzyme and acetylcholine substrate. This ITO-EGFET can be used to detect carbaryl in concentration rage 0.001 - 1 mM by using the ITO-EGFET without enzyme as control condition. This work shows possibility of ITO-EGFET for application in pesticide detection with an easy substrate preparation, low cost and can be applied in field used with others pesticides.