Now showing 1 - 10 of 12
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    Conjugation of antibody on gold nanoparticles for biosensors application
    (2020-01-01)
    Ta-Aithuak, Sarocha
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    Loedsapchinda, Naruenard
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    Regarding to the unique optical property and biocompatible, gold nanoparticles have been widely used to functionalize with antibodies probe for testing specificity with their antigen targets. The antibody immobilized onto gold nanoparticles which synthesized by different methods were studied. Gold nanoparticles synthesized by citrate reduction method and by using poly(ethylene)glycol (PEG) coated gold nanoparticles were immobilized with IgM antibody by physical adsorption. Gold nanoparticles before and after functionalize with antibodies were characterized with fourier-transform infrared spectroscopy (FTIR) for functional group and UV-Vis spectroscopy for absorption wavelength. Finally, a specificity test was conducted using spot of anti-IgM antibody onto nitrocellulose membrane to confirm the bioactivity of antibodies attached to gold nanoparticles.
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    Feasibility of Prediction Model for Internal Tumor Target Volume from 4-D Computed Tomography of Lung cancer
    (2021-01-01)
    Puangragsa, Utumporn
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    Lomvisai, Pitchayakorn
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    Puangragsa, Sarut
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    Setakornnukul, Jiraporn
    4-Dimensional computed tomography (4DCT) is the most common technique to determine organ movement due to breathing motion. However, the ability of 4DCT to acquire CT images as a function of the respiratory phase increases higher radiation dose. To reduce the patient's radiation dose, this study created lung motion prediction models used to estimate tumor target movement in ten respiratory phases by detecting only external organ movement during a complete respiration cycle without radiation with Kinect. The average overall amplitude difference between RPM and Kinect signals in the phantom experiment was 0.02 ± 0.1 mm. F1 score of 100% for all most all classifications except classification 2,3,6,7 and 8 of 85%,83%,90%, 84%,85% where irregular breathing pattern. Essentially, the proposed tumor movement scheme's total accuracy (average of F1 scores) is 92.7 %. Deep learning model can predict tumor motion range and classification zone by used detection of the external respiratory signal
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    Synthesis of Zeolite Y from Kaolin via hydrothermal method
    (2019-01-01)
    Krongkrachang, Pimpetch
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    Thungngern, Pimpreeya
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    Asawaworarit, Panuruj
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    ;
    ZeoliteY has been successfully synthesized via a two-step hydrothermal method with kaolin from Lampang province as a precursor. For the first hydrothermal, kaolin with an addition of sodium silicate (NaSi<inf>3</inf>O<inf>7</inf>) or tetraethyl orthosilicate (TEOS) was mixed with sodium hydroxide (NaOH) and autoclaved for 200<sup>o</sup>C at different reaction times for 6, 8 and 10 h, respectively. Sodium hydroxide and hydrochloric acid were added into the compound mixture to adjust its pH before it was formed into gel. After a second time hydrothermal at 90°C for 2 days, the solid product was filtered out, washed by deionized water and dried overnight. The zeolite was then characterized by X-ray diffraction spectroscopy (XRD) to identify type and crystallization, scanning electron microscopy (SEM) for morphology, Fourier transform infrared spectroscopy (FTIR) to identify its functional group and N<inf>2</inf> adsorption to study the adsorption/desorption isotherm and pore distribution. From N<inf>2</inf> adsorption-desorption isotherm, it is revealed that the zeolite obtained by this method have 571 m<sup>2</sup>/g surface area and pore size distribution around 4.89 nm.
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    Study on detection of carbaryl pesticides by using surface-enhance raman spectroscopy
    (2020-01-01)
    Chakaja, Chaiwat
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    Limwichean, Saksorn
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    Nuntawong, Noppadon
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    Eiamchai, Pitak
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    Kalasung, Sukon
    In this research, the Ag nanorod structure was used as surface enhanced Raman scattering (SERS) chip which provides a sensitive detection signal for trace analysis of carbaryl pesticide. Carbaryl in solid form was measured by using the standard Raman spectroscopy to investigate the spectrum. Carbaryl at various concentrations was prepared in acetonitrile and dropped on the SERS chip for measuring Raman spectrum by a portable Raman spectrometer. The measurement condition including laser power and exposure time were studied to test the performance of SERS chip for carbaryl detection. From the results, the SERS chip useful for enhancing the Raman scattering signal which was increased depending on the laser power and exposure time. Carbaryl can be detected on SERS chip couple with the portable Raman spectrometer with the limit of detection of 10<sup>-5</sup> M.
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    Item type:Publication,
    Zeolite P from kaolin via hydrothermal method
    (2018-09-05) ;
    Amnaphiang, Phanwasa
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    Asawaworarit, Panuruj
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    Chollacoop, Nuwong
    Zeolite P has been successfully synthesized from natural kaolin via two step hydrothermal process. The natural kaolin from Lampang, Thailand was studied for this research. In first hydrothermal, kaolin was mixed with sodium hydroxide solution at 200 <sup>o</sup>C for different reaction times from 3 - 5 hours, respectively.Sodium hydroxide and hydrochloric acid were added into the mixture to adjust their pH before they were formed into gel. Second hydrothermal process, the gel was kept of 90 <sup>o</sup>C for 3 days to obtain zeolite P. Synthesized zeolite P was characterized by X-ray diffraction (XRD) for identification the type and crystallization. Besides, the morphology was characterized by scanning electron microscopy)SEM.(The functional group was characterized by Fourier-transform infrared spectroscopy (FTIR). From this study, it was found that the increasing of reaction time in first hydrothermal activation and hydrochloric acid concentration lead to the high quality of zeolite P.
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    Breast Cancer Detection using IR-UWB with Deep Learning
    (2021-01-01)
    Khumdee, Mawin
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    Assawaroongsakul, Pongpol
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    This paper proposes breast cancer positioning detection using the IR-UWB system with deep learning, which is an interesting alternative method. When compared to ultrasound, x-ray mammogram, and CT-scan, there are several advantages to using IR-UWB, including low cost, less energy required, less long-term effect, portability, and providing much more breast cancer screening access for patients. Nowadays, the IR-UWB system has many techniques for processing IR-UWB signals, and one of the most interesting technique is using deep learning. In this study, we collected data from nine IR-UWB antennas. Then, the prepared data is fed through Deep Neural Networks to find the hidden patterns of signal and predict the cancer position which are 16 of breast cancer positions and one of undetected, also known as 17 classes. The model gave an average accuracy up to 95.60%.
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    Rapid detection of cypermethrin by using surface-enhanced raman scattering technique
    (2020-01-01)
    Leung, Wipawanee
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    Limwichean, Saksorn
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    Nuntawong, Noppadon
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    Eiamchai, Pitak
    ;
    Kalasung, Sukon
    Cypermethrin is a toxic pesticide in the pyrethroid group. A Surface Enhanced Raman Scattering (SERS) based sensor has been developed to achieve simple pesticide sensing. In this work, rapid detection of cypermethrin by using the handheld Raman spectroscopy coupled with SERS substrate was demonstrated. SERS-active silver nanorods substrate was used to enhance Raman signals of test samples. The effect of exposure time and drop volume of sample was studied for cypermethrin measurement. The results found that the silver nanorods substrate can be used to measure cypermethrin in the range of 10<sup>-6</sup> to 10<sup>-3</sup> M with a handheld Raman spectrometer. Furthermore, the Raman signal of cypermethrin was confirmed by measuring solid cypermethrin with the standard Raman spectrometer. SERS substrate was competent to detect cypermethrin with a limit of detection (LOD) of 10<sup>-6</sup> M.
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    Item type:Publication,
    Gold nanorod-optical fiber for sensing biomolecular interaction
    (2020-01-01)
    Chueaiarrom, Channarong
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    Loedsapchinda, Naruenard
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    Puttharugsa, Chokchai
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    Pipatpanukul, Chinnawut
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    Srikhirin, Toemsak
    The optical fiber deposits with gold nanorods was successfully accomplished as a biosensor device which sensing to the refractive index change on the surrounding surface. The principle is based on the localized surface plasmon resonance (LSPR) occurs on the metal surface of gold nanorods (AuNRs). The gold nanorods were coated on the glass core fiber surface through the covalent bond. The uncladded area fiber optic was incubated in 5% of (3-Mercaptopropyl) trimethoxysilane (MPTMS) in ethanol for overnight to alter the thiol group on the surface. The gold nanorods with aspect ratio ⁓2.035 was deposited on thiol surface by incubation the optical fiber in gold nanorod solution for 6 hr. The sensor provides the sensitivity of 4.94×104 a.u./RIU. Moreover, antibody for blood grouping was immobilized on the AuNRs-optical fiber to detect the red blood cells. The AuNRs-optical fiber can detect A antigen on the immobilized anti-A surface with Δ%T of 0.98 and can detect B-antigen on the immobilized anti-B surface with Δ%T of 1.78. This work possesses possibility of a simple AuNRs-optical fiber to detect the biomolecular interaction. The authors would like to thank Thailand Center of Excellence in Physics for financial support.
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    Extended-gate field effect transistor (EGFET) for carbaryl pesticide detection based on enzyme inhibition assay
    (2017-01-01)
    Sasipongpana, Supanat
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    Rayanasukha, Yossawat
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    Prichanont, Seeroong
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    Thanachayanont, Chanchana
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    Porntheeraphat, Supanit
    An extended - gate filed effect transistor (EGFET) is a device which can be applied to chemical sensing devices. In this study, EGFET was constructed by using an indium tin oxide (ITO) glass substrate. The small piece ITO with sensing area around 3x3 mm<sup>2</sup> was used as carbaryl pesticide sensor based on enzyme inhibition assay. The prepared ITO-EGFET sensors were test with pH solutions to obtain sensitivity compared with commercial ion sensitive field effect transistor (ISFET). Preparation of ITO substrate for enzyme inhibition assay by dropped acetylcolinesterase enzyme at 0.5 Unit which were trapped in an agarose gel matrix onto substrates. This prepared ITO acts as extended gate sensing membrane for EGFET. The amounts of drop volume of enzyme were varied to obtain the highest signal for reaction with acetylcholine substrate. Cabaryl can inhibit enzyme function resulting in the decreasing signal of interaction between enzyme and acetylcholine substrate. This ITO-EGFET can be used to detect carbaryl in concentration rage 0.001 - 1 mM by using the ITO-EGFET without enzyme as control condition. This work shows possibility of ITO-EGFET for application in pesticide detection with an easy substrate preparation, low cost and can be applied in field used with others pesticides.
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    Deep Learning-Based Human Recognition Through the Wall using UWB radar
    (2021-01-01)
    Assawaroongsakul, Pongpol
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    Khumdee, Mawin
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    Human activity detection in obscured or invisible area, for instance, human detection through the wall has become an interesting topic because it has potential for security, rescue, activity analysis application, etc. UWB radar, a detection system produces short radio frequency pulses and measures the reflected signals which UWB pulses have high spatial resolution and enable penetration in dielectric materials, was used to collect human activity through the wall signals at the frequency range of 3 GHz in this research. Subsequently, we applied signal data with the Deep Neural Network model to classify 5 classes of human activity including standing, walking, sitting, laying, and no-human gave the F1 score up to 96.94%.