Rungjindamai, Nattawut

Loading...
Thumbnail Image
Preferred name
Rungjindamai, Nattawut
Alternative Name
Rungjindamai, N.
Main Affiliation
Email
nattawut.ru@kmitl.ac.th

Settings

Now showing 1 - 2 of 2
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    A novel strategy to reduce overwintering inoculum of Monilinia laxa
    (2014-10-02)
    Rungjindamai, Nattawut
    ;
    Jeffries, Peter
    ;
    Xu, Xiang Ming
    Brown rot on cherry and plum, caused by Monilinia laxa, is an important disease, for which overwintered mummified fruit is a significant inoculum source for infection of flowers and fruit in the spring. Experiments were conducted to assess the potential of applying plant protection products in winter and/or early spring to suppress sporulation on mummified fruit. Products tested included Indar 5 EW (a commercial fungicide, a.i. fenbuconazole), Aureobasidium pullulans Y126 [a candidate biocontrol agent (BCA)]), Bacillus sp. B91 (a candidate BCA) and Serenade (a commercially formulated BCA of Bacillus subtilis strain QST 713) in addition to control treated with tap water. Indar and A. pullulans Y126 significantly reduced sporulation when applied once in winter. Overall, a single treatment in early spring (February) was slightly more effective than single treatment in winter (November). Application of all products in both winter and early spring led to significant reduction in sporulation. Indar had the highest efficacy, reducing the number of spores from 9 × 10<sup>5</sup> to 5 × 10<sup>3</sup> per mummified plum when applied twice. Of the three BCAs applied on both occasions, A. pullulans Y126 had the highest efficacy, reducing the number of spores from 9 × 10<sup>5</sup> to 5 × 10<sup>4</sup> per mummified plum. There were no synergistic but additive effects between the two applications in winter and early spring based on the Bliss independence test. These results suggest that reducing overwintering inoculum in dormant season is effective and may be part of an integrated management strategy for brown rot on stone fruit.
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Biological Control of Pichia bruneiensis AL3 against Green Mould Disease on Oranges
    (2024-01-01)
    Phankaeo, N.
    ;
    Nuchthien, K.
    ;
    Pitthayajirakul, N.
    ;
    Rungjindamai, N.
    Agriculture plays an essential role in Thailand economy. However, to control plant pathogens and maximize crop yield, pesticides are overused and misused. This results in toxic pollutants that are harmful to humans and the environment. Biological control offers an alternative to the use of synthetic chemicals. Mandarin orange (Citrus reticulata) is widely grown and consumed in Thailand, but it is threatened by green mould disease caused by Penicillium digitatum, which usually occurs at post-harvest storage. A total of 40 isolates of epiphytic yeasts were isolated from symptomless orange peels. They were screened for antagonistic activity against P. digitatum originally isolated from a diseased orange using a dual culture method. Five isolates exhibited at least 70% inhibition; therefore, they were further screened for their activity on orange using fruit inoculation. An isolate, AL3, reduced the disease incidence (79%) and lesion size (34.75±2.57 mm) caused by P. digitatum compared to the control treatments (disease incidence, 100% and lesion size, 50.00±1.03 mm, respectively). The yeast was analyzed using molecular data based on Internal Transcribed Spacers (ITS region) and it was identified as Pichia bruneiensis AL3. Its mode of action on anti-sporulation was studied. When tested on agar plates, living cells and culture broth of P. bruneiensis AL3 were able to inhibit spore germination of P. digitatum resulting in 33.2 and 38.2% germination, respectively, compared to the control treatment (76.8% germination). These results showed that P. bruneiensis AL3 has the potential to develop as a biological control agent (BCA).