Rahong, Sakon

Loading...
Thumbnail Image
Preferred name
Rahong, Sakon
Alternative Name
Rahong, S.
Main Affiliation
Email
sakon.ra@kmitl.ac.th

Settings

Now showing 1 - 2 of 2
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Annealed zno/al2o3 core-shell nanowire as a platform to capture rna in blood plasma
    (2021-07-01)
    Takahashi, Hiromi
    ;
    Yasui, Takao
    ;
    Klamchuen, Annop
    ;
    Khemasiri, Narathon
    ;
    Wuthikhun, Tuksadon
    RNA analytical platforms gained extensive attention recently for RNA-based molecular analysis. However, the major challenge for analyzing RNAs is their low concentration in blood plasma samples, hindering the use of RNAs for diagnostics. Platforms that can enrich RNAs are essential to enhance molecular detection. Here, we developed the annealed ZnO/Al<inf>2</inf>O<inf>3</inf> core-shell nanowire device as a platform to capture RNAs. We showed that the annealed ZnO/Al<inf>2</inf>O<inf>3</inf> core-shell nanowire could capture RNAs with high efficiency compared to that of other circulating nucleic acids, including genomic DNA (gDNA) and cell-free DNA (cfDNA). Moreover, the nanowire was considered to be biocompatible with blood plasma samples due to the crystalline structure of the Al<inf>2</inf>O<inf>3</inf> shell which serves as a protective layer to prevent nanowire degradation. Our developed device has the potential to be a platform for RNA-based extraction and detection.
  • Loading...
    Thumbnail Image
    Some of the metrics are blocked by your 
    consent settings
    Item type:Publication,
    Mutation detection of urinary cell-free DNA via catch-and-release isolation on nanowires for liquid biopsy
    (2023-08-15)
    Takahashi, Hiromi
    ;
    Yasui, Takao
    ;
    Hirano, Masaki
    ;
    Shinjo, Keiko
    ;
    Miyazaki, Yusuke
    Cell-free DNA (cfDNA) and extracellular vesicles (EVs) are molecular biomarkers in liquid biopsies that can be applied for cancer detection, which are known to carry information on the necessary conditions for oncogenesis and cancer cell-specific activities after oncogenesis, respectively. Analyses for both cfDNA and EVs from the same body fluid can provide insights into screening and identifying the molecular subtypes of cancer; however, a major bottleneck is the lack of efficient and standardized techniques for the isolation of cfDNA and EVs from clinical specimens. Here, we achieved catch-and-release isolation by hydrogen bond-mediated binding of cfDNA in urine to zinc oxide (ZnO) nanowires, which also capture EVs by surface charge, and subsequently we identified genetic mutations in urinary cfDNA. The binding strength of hydrogen bonds between single-crystal ZnO nanowires and DNA was found to be equal to or larger than that of conventional hydrophobic interactions, suggesting the possibility of isolating trace amounts of cfDNA. Our results demonstrated that nanowire-based cancer screening assay can screen cancer and can identify the molecular subtypes of cancer in urine from brain tumor patients through EV analysis and cfDNA mutation analysis. We anticipate our method to be a starting point for more sophisticated diagnostic models of cancer screening and identification.