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    Changes in ascorbic acid, total polyphenol, phenolic acids and antioxidant activity in juice extracted from coated kiew wan tangerine during storage at 4, 12 and 20°c
    (2010-03-01) ;
    Haruenkit, Ratiporn
    The aim of this research was to investigate changes in ascorbic acid, total polyphenol, phenolic acids and antioxidant activity in juice extracted from Kiew Wan tangerines (Citrus reticulate Blanco cv. Kiew Wan) coated with shellac. The coated fruits were stored for five weeks at 4, 12 and 20°C and samples were taken weekly for analysis. Ascorbic acid decreased during storage at all tested temperatures. The major phenolic acids found were ferulic acid followed by sinapic, caffeic and p-coumaric acid. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2 azinobis (3-ethylbenzothiazoline-6-sulfonate (ABTS) antioxidant activities increased during storage and were correlated with total polyphenol and phenolic acid contents. Sinapic, caffeic and p-coumaric acid levels were significantly correlated with the DPPH while ferulic acid was correlated with the ABTS. All phenolic acids increased during the early stage of storage at 4 and 12°C but decreased at the end. Weight loss of coated fruit during storage was in the range of 4-17%.
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    Item type:Publication,
    Formulation of chitosan-oleic acid coating for Kiew Wan tangerine by response surface methodology
    (2010-05-01) ;
    Haruenkit, Ratiporn
    The coating formulation is an essential factor in maintaining the postharvest quality of tangerines. A common problem found in coated tangerines is off-flavor due to improper coating. The aim of this study was to use chitosan and oleic acid to formulate the most suitable coating for tangerines displayed at room temperature (30±2°C). In order to optimize the formulation, response surface methodology (RSM) with a central composite design was applied. The response effect of chitosan (1-2%) and oleic acid (1-4%) on weight loss, ethanol in juice, and carbon dioxide and oxygen in fruit were investigated. A second order model was used to explain the effect of the dependent variables (weight loss, O<inf>2</inf>, CO<inf>2</inf>, ethanol), which were highly significant and produced R<sup>2</sup> values in the range from 77 to 92%. The optimum coating formulation for tangerine was found to be 1% chitosan (v/v) and 2.5% oleic acid (v/v), which was based on a composite desirability value of 0.80.
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    Item type:Publication,
    Subchronic toxicity of liquid smoke from 'Tian Op' in Wistar rats
    (2013-07-18)
    Watcharananun, Wanwarang
    ;
    Chivapat, Songpol
    ;
    Rangsripipat, Anudep
    ;
    Attawish, Aimmanas
    ;
    Chavalittumrong, Pranee
    Liquid smoke (LS) from 'Tian Op ' has been developed for 'feathering' (softening) the odor of many Thai desserts. The benefits of liquid smoke are reduced smoking time, increased ease of utilization and standardization of the intensity of the flavor in dessert products. The Medicinal Plant Research Institute, Department of Medical Science, Ministry of Public Health, Thailand conducted a 90 d oral subchronic toxicity test of LS in 120 Wistar rats divided into five groups (12 per sex per group). Groups 1 and 2 were control groups receiving distilled water and propylene glycol at a volume of 10 mL.kg<sup>-1</sup>. day<sup>-1</sup>, respectively, where the weight was based on live body weight. Groups 3-5 were experimental groups receiving LS at doses of 0.04, 0.4 and 4.0 g.kg<sup>-1</sup>.day<sup>-1</sup>, respectively. LS at any dosage did not affect growth, food consumption and hematological values. Rats receiving LS at doses of 0.4 and 4.0 mL.kg<sup>-1</sup>.day<sup>-1</sup> had significantly higher albumin levels than the water control and the propylene glycol-treated rats, but these alterations did not indicate any abnormalities. Histopathology of organs revealed no abnormalities related to liquid smoke toxicity. Therefore, it may be concluded that liquid smoke at the given dosages did not produce toxicity in Wistar rats.