Nukeaw, Jiti

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Nukeaw, Jiti
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Nukeaw, J.
Nukeaw, Jitti
Nukeaw, Jiji
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jiti.nu@kmitl.ac.th

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    Item type:Publication,
    A disposable polydimethylsiloxane microdevice for DNA amplification
    (2010-02-05)
    Lekwichai, A.
    ;
    Porntheeraphat, S.
    ;
    Bunjongpru, W.
    ;
    Sripumkhai, W.
    ;
    Supadech, J.
    In this study, we demonstrate the disposable polydimethylsiloxane (PDMS) microchip provided for DNA amplification. The device consists of two main parts. The first part is PDMS/glass stationary chamber, the other part is a temperature-control microdevice on SiO<inf>2</inf>/Si substrate. This device consists of a thin film Pt-microheater and a Pt-temperature sensor, which were fabricated with CMOS compatible process. The performance of the device in the DNA amplification shows that, with 10 μl of PCR mixture volume, the approximately 700 bp DNA were successfully amplified within 50 minutes by 30 PCR cycles. The amplified products were comparable with those of a conventional method using electrophoresis. The PCR chip is also suitable for mass production. © (2010) Trans Tech Publications.