Nukeaw, Jiti

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Nukeaw, Jiti
Alternative Name
Nukeaw, J.
Nukeaw, Jitti
Nukeaw, Jiji
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Email
jiti.nu@kmitl.ac.th

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    Item type:Publication,
    A disposable polydimethylsiloxane microdevice for DNA amplification
    (2010-02-05)
    Lekwichai, A.
    ;
    Porntheeraphat, S.
    ;
    Bunjongpru, W.
    ;
    Sripumkhai, W.
    ;
    Supadech, J.
    In this study, we demonstrate the disposable polydimethylsiloxane (PDMS) microchip provided for DNA amplification. The device consists of two main parts. The first part is PDMS/glass stationary chamber, the other part is a temperature-control microdevice on SiO<inf>2</inf>/Si substrate. This device consists of a thin film Pt-microheater and a Pt-temperature sensor, which were fabricated with CMOS compatible process. The performance of the device in the DNA amplification shows that, with 10 μl of PCR mixture volume, the approximately 700 bp DNA were successfully amplified within 50 minutes by 30 PCR cycles. The amplified products were comparable with those of a conventional method using electrophoresis. The PCR chip is also suitable for mass production. © (2010) Trans Tech Publications.
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    Study of PDMS compounds using the adhesion force determined by AFM force distance curve measurements
    (2010-02-05)
    Vanitparinyakul, S.
    ;
    Pattamang, P.
    ;
    Chanhom, A.
    ;
    Tunhoo, B.
    ;
    Thiwawong, T.
    The atomic force microscope(AFM) was used to perform surface force measurements in contact mode to investigate surface properties of model systems at the nanoscale. Three different Polydimethylsiloxane (PDMS) compounds were observed. The first consisted of pure PDMS, the second of PDMS blend with the nanoparticles Zinc Oxide(PDMS/ZnO) and the third of PDMS blend with the nanoparticles Zinc Oxide and toluene solvent(PDMS/ZnO/toluene), respectively. Surface morphology and the adhesion force were investigated by using atomic force microscopy. Force - distance curve measurement was performed in a contact mode, which used tip as silicon nitride. Moreover, we found a significantly different of the adhesion force when modified by nanoparticles ZnO and toluene solvent. © (2010) Trans Tech Publications.