Lerkkasemsan, Nuttapol

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Lerkkasemsan, Nuttapol
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Email
nuttapol.le@kmitl.ac.th

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    Item type:Publication,
    Application of Endoxylanases of Bacillus halodurans for Producing Xylooligosaccharides from Empty Fruit Bunch
    (2023-01-01)
    Thirametoakkhara, Chanakan
    ;
    Hong, Yi Cheng
    ;
    Lerkkasemsan, Nuttapol
    ;
    Shih, Jian Mao
    ;
    Chen, Chien Yen
    Endo-1,4-β-xylanase catalyzes the random hydrolysis of β-1,4-D-xylosidic bonds in xylan, resulting in the formation of oligomers of xylose. This study aims to demonstrate the promise of endoxylanases from alkaliphilic Bacillus halodurans for the production of xylooligosaccharides (XOS) from oil palm empty fruit bunch (EFB) at high pH. Two enzyme preparations were employed: recombinant endoxylanase Xyn45 (GH10 xylanase) and nonrecombinant endoxylanases, a mixture of two extracellular endo-1,4-β-xylanases Xyn45 and Xyn23 (GH11 xylanase) produced by B. halodurans. EFB was first treated with an alkaline solution. Then, the dissolved xylan-containing fraction was retained, and a prepared enzyme was added to react at pH 8 to convert xylan into XOS. Compared with the use of only Xyn45, the combined use of Xyn45 and Xyn23 resulted in a higher yield of XOS, suggesting the synergistic effect of the two endoxylanases. The yield of XOS obtained from EFB was as high as 46.77% ± 1.64% (w/w), with the xylobiose-to-xylotriose ratio being 6:5. However, when the enzyme activity dose was low, the product contained more xylotriose than xylobiose. Four probiotic lactobacilli and bifidobacteria grew well on a medium containing XOS from EFB. The presence of XOS increased cell mass and reduced pH, suggesting that XOS promoted the growth of probiotics.
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    Life cycle assessment of diuron from cradle to grave: case study in agave farm
    (2019-11-01)
    Thirametoakkhara, Chanakan
    ;
    Lerkkasemsan, Nuttapol
    Diuron is a herbicide commonly used in agriculture to control and kill pre-emergent weeds. Extensive use of the chemical nonetheless harms the environment and human health. This research thus investigates the cradle-to-grave environmental impacts and risks to human health and ecosystem of diuron production, consumption, and disposal using life cycle assessment. The research focused on five production schemes (schemes A–E) of varying origins of raw materials and countries. Simapro 8.5 was used to simulate the environmental impacts (midpoint impacts) and risks to human health and ecosystem (endpoint impacts). The results revealed a positive correlation between overall environmental impact and transportation distance, rendering scheme C the most environmentally-friendly option due to the shortest transportation distance. Likewise, the endpoint-impact results indicated scheme C as the most favorable scheme, given the lowest overall human health risk and ecosystem damage.