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Effect of actinomycin D isolated from the cultured broth of marine streptomyces spp. On cell division protein FtsZ
Author(s)
Charoenwiwattanakij, Phennapa
Pratuangdejkul, Jaturong
Chongruchiroj, Sumet
Suwanborirux, Khanit
Chingunpitak, Jiraporn
Satitpatipan, Veena
Date Issued
January 1, 2020
Type
Article
Abstract
In the course of our investigation on antibacterial substances with FtsZ inhibitory effect, actinomycin D was isolated from the ethyl acetate extract of Streptomyces sp. LT3-17. The compound showed antibacterial activities. The MIC values of actinomycin D against Staphylococcus aureus ATCC 25923, Methicillin-resistant Staphylococcus aureus DMST 20654, Bacillus subtilis ATCC 6633, Escherichia coli ATCC 25922, and Pseudomonas aeruginosa ATCC 27853 were 3.13, 0.39, 0.10, 300.00, and 500.00 µg/mL, respectively. The morphology study of E. coli JW0093 treated with the compound showed inhibitory effect on cell elongation. The inhibition of FtsZ activity by actinomycin D was shown as the inhibition of GTPase activity with IC50 value, 20.06 µM. The polymerization of E. coli FtsZ protein (EcFtsZ) treated with 0.01 µM actinomycin D showed the degree of polymerization ratio was less than 1.0 indicating the inhibitory potential of actinomycin D on FtsZ polymerization. In silico study was performed to predict binding mode of actinomycin D into nucleotide binding pocket of the homology model of EcFtsZ protein. From the results of these experiments, the isolation and elucidation methods of actinomycin D as well as its novel mechanism as FtsZ inhibitors have been discovered.
Citation
Chiang Mai Journal of Science, 47(3), 362-377, 2020