Promicromonospora thailandica sp. nov., isolated from marine sediment

Thawai, Chitti; Kudo, Takuji

doi:10.1099/ijs.0.035972-0

Promicromonospora thailandica sp. nov., isolated from marine sediment

Author(s)

Thawai, Chitti

Kudo, Takuji

Date Issued

September 1, 2012

Type

Article

DOI

10.1099/ijs.0.035972-0

Abstract

A novel actinomycete, strain S7F-02^T, which produced primary branched hyphae and fragmented into V- and Y-shaped bacillary cells, was isolated from marine sediment collected in the Andaman Sea, Trang Province, Thailand. Lysine was found to be the diagnostic diamino acid in the cell-wall peptidoglycan. The whole-cell sugars of strain S7F-02^T were ribose, arabinose and glucose. The characteristic phospholipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The predominant menaquinone was MK-9(H4). The predominant cellular fatty acids were anteiso-C15: 0 and iso-C15: 0. The G+C content of the genomic DNA of strain S7F-02^T was 70.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain S7F-02^T should be classified in the genus Promicromonospora. This strain formed a close association with Promicromonospora citrea DSM 43110^T, with which it shared 99.2% 16S rRNA gene sequence similarity. DNA-DNA hybridization data together with physiological and biochemical properties showed that strain S7F-02^T could be readily distinguished from its closest phylogenetic relatives. On the basis of these phenotypic and genotypic data, strain S7F-02^T is considered to represent a novel species of the genus Promicromonospora, for which the name Promicromonospora thailandica sp. nov. is proposed. The type strain is S7F-02^T (=BCC 41922^T=JCM 17130^T). © 2012 IUMS Printed in Great Britain.

Citation

International Journal of Systematic and Evolutionary Microbiology, 62(9), 2140-2144, 2012