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Genetic mapping of leaf blast resistance gene in landrace rice cultivar ‘GS19769’
Author(s)
Date Issued
August 31, 2015
Type
Article
Abstract
The rice blast fungus, Magnaporthe oryzae, is highly varied and therefore overcomes resistance in a few years. Rice cultivars with the ability to resist different blast races are therefore required. In this study, a resistance gene of landrace rice cultivar ‘GS19769’ is identified using a mix of 19 blast isolates collected from several epidemic areas in Thailand. The selected cultivar was fertilised with the blast susceptible variety, Khao Dok Mali 105 (KDML105), to generate the mapping population. Segregation analysis in the F2 population shows that ‘GS19769’ contains more than 1 resistance gene, as the Chi-square test for segregation of resistance and susceptibility does not fit the ratio of 3:1. The bulk segregant analysis by simple sequence repeat (SSR) markers shows that the identified resistance gene is linked to the SSR markers RM224 and RM144 on chromosome 11. Analysis of 15 F2 plants susceptible to the blast was conducted. The gene was mapped to RM224 and RM144 at the same distance of 20 cM.
Citation
Maejo International Journal of Science and Technology, 9(2), 278-287, 2015