Loading...
Quantitation of three strecker aldehydes from enzymatic hydrolyzed rice bran protein concentrates as prepared by various conditions
Author(s)
Date Issued
June 28, 2019
Type
Article
Abstract
The quantitation of three Strecker aldehydes-2-methypropanal (2-MP), 2-methylbutanal (2-MB), and 3-methylbutanal (3-MB)-from rice bran protein hydrolysate (RBPH) prepared under various conditions were investigated. The preparation conditions included hydrolysis time (0, 0.25, 2, 4, or 8 h), pH adjustment (pH 4.0, 7.0, or 10.0), and sugar addition (sucrose, glucose, or fructose). These conditions provide a significant potential for aroma generation from the Strecker degradation and Maillard reaction. The Strecker aldehyde quantitation was performed using gas chromatography (GC) with cryo-focusing technique. These combined techniques encourage the precise 2-MB and 3-MB quantitation. The highest concentrations of three Strecker aldehydes were found in RBPH that was prepared by alcalase hydrolysis at 4 h with fructose addition (RBPH-F) and adjusted to pH 7.0 before spray drying. Thirty-nine aroma-active compounds of RBPH-F were discovered using solid-phase microextraction coupled with GC-olfactometry.
Citation
Journal of Agricultural and Food Chemistry, 67(29), 8205-8211, 2019