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An E. coli screening machine in water via fluorescence detection
Author(s)
Juhong, Aniwat
Date Issued
January 10, 2019
Type
Conference Paper
Abstract
We have developed a fluorescence based detection for rapid E. coli which is an indicator for fecal contamination for water quality. This technology has cut down the time taken to detect E. coli from a few days to just a couple of hours. The system relies on the enzyme β-D glucuronidase (GUD) reacts with 4-methylumblliferyl-β-D glucuronide (MUG) resulting in a byproduct named 4-methylumbellliferone (4MU). Our system can detect this byproduct in the concentration range from 0.01 to 80 μM which can then be enumerated to be the number of E. coli.
Citation
Bmeicon 2018 11th Biomedical Engineering International Conference, 2019