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DILUTE ACID HYDROLYZED SOYBEAN MEAL FOR CULTIVATION OF ISOLATED CLOSTRIDIUM SP. G10
Author(s)
Sabua, Chonthicha
Hemnusoornnanon, Nutcha
Nuangpanom, Phornnapa
Leksawasdi, Noppol
Date Issued
January 1, 2022
Type
Conference Paper
Abstract
Soybean meal is a by-product from soybean oil manufacturing. It contained, on the mass basis, crude fiber (6.3%) and protein (47.6%) after oil extraction. The aim of study was to investigate the cultivation of isolated Clostridium sp. G10 using soybean meal hydrolysate as a N-and C-source in fermentation medium. Soybean meal hydrolyzed with 0.02 to 0.1 M of HCl and H2SO4 and was heated at 121 °C, 15 psi for 10-30 min. The result showed that hydrolyzing soybean with 0.1 M HCl and 20-min heating period were the optimal conditions to obtain soluble protein (24.45 ±2.21 g/L) and reducing sugar (13.12 ±1.09 g/L). To study the effect of N-and C-sources replacement in T6 medium, soybean meal hydrolysate was utilized to replace yeast extract and tryptone resulting in 8 g/L soluble protein and used as a C-source with the addition of glucose with 50 g/L reducing sugar equivalent. The butanol concentration (10.80 ±0.44 g/L) was slightly lowered than that of control T6 medium (12.15 ±0.07 g/L). These results demonstrated that Clostridium sp. was able to uptake the N-and C-sources contained in the hydrolyzed soybean meal. However, a C-source alternative is required to mitigate the cost of raw materials.
Citation
European Biomass Conference and Exhibition Proceedings, 927-931, 2022