Loading...
Sinosporangium siamense sp. nov., isolated from soil and emended description of the genus Sinosporangium
Author(s)
Suriyachadkun, Chanwit
Ngaemthao, Wipaporn
Chunhametha, Suwanee
Sanglier, Jean Jacques
Kitpreechavanich, Vichien
Date Issued
January 1, 2014
Type
Article
Abstract
An actinomycetes strain A-T 1946T that developed spherical sporangia containing non-motile spores on aerial mycelia was isolated from dry deciduous forest soil in Thailand. 16S rRNA gene sequence analysis indicated that strain A-T 1946T belongs to the genus Sinosporangium, being closely related to Sinosporangium album 6014T (98.8 % sequence similarity). The DNA-DNA relatedness values were 43.7-50.9 %, which were significantly below 70 % and differentiated strain A-T 1946T from the closest species. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained rhamnose, ribose, madurose and glucose. The predominant menaquinone was MK-9(H2). The diagnostic phospholipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, lysophosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol-mannoside, N-acetylglucosamine-containing phospholipids, two unknown phosphoglycolipids and two unknown phospholipids. The predominant cellular fatty acids were unsaturated C16: 1 and C17: 1, and saturated C16: 0 and 10-methyl-C17: 0. Following an evaluation of phenotypic, chemotaxonomic and genotypic characteristics, the isolate is proposed to represent a novel species of genus Sinosporangium to be named Sinosporangium siamense sp. nov. The type strain is A-T 1946T (= BCC 29081T = NBRC 109515T). An emended description of the genus Sinosporangium is also provided. © 2014 IUMS.
Citation
International Journal of Systematic and Evolutionary Microbiology, 64(PART 8), 2828-2833, 2014