Liquid and vapor-phase vinegar reduces Klebsiella pneumoniae onfresh coriander

Abstract

Samples of fresh coriander were sourced from several markets in the Ladkrabang and Minburi areas of Bangkok, Thailand. These were examined in the laboratory for fecal coliform contamination. The bacterium Klebsiella pneumoniae was the predominant contaminant in 48.5-84.0% of the 30 samples tested. Inhibition of surface contaminations of K.pneumoniae by vinegar was measured invitro for either liquid-or vapor-phase exposures. For liquid-phase exposure, levels above 2.4% (v/v) acetic acid (AA) completely inhibited K.pneumoniae. After 50min of vapor-phase exposure, 8% AA completely inhibited K.pneumoniae spread on Mueller Hilton agar (4.10±0.04logCFU/ml, 30±2°C, 80±2% RH). Reduction of K.pneumoniae contamination on fresh coriander leaves using vinegar vapor was also examined. The effectiveness of inhibition was related to the level of K.pneumoniae inoculation, with vinegar vapor being shown to be an effective control agent for surface contaminations on fresh coriander so reducing the potential health risks to consumers.