A Study of Oyster Mushrooms for Producing Cellulase Enzyme

Abstract

The objective of this research was to study three species of Pleurotus spp., are widely consumed edible mushrooms, consisting of P. sajor-caju, P. ostreatus and P. cystidiosus. Basidiocarp and spawn on sorghum seeds were collected from seven provinces in Central and Eastern of Thailand. Thirteen isolates were recovered from 15 samples which include P. sajor-caju, P. ostreatus and P. cystidiosus for 5, 6 and 2 isolates, respectively. The studies of their growth rates and enzymatic capability were performed on potato dextrose ager (PDA) and Czapek Dox Agar mixed with 1% carboxymethyl cellulose (CZ+CMC), respectively. Both media were incubated at 25ºC for seven days. The three most potential isolates were selected including P. sajor-caju PS5, P. ostreatus PO7 and P. cystidiosus PC1. Therefore, these mushrooms have potential for development in the future. Because they can grow rapidly and produce cellulase effectively.