Influence of plant growth regulators on shoot development of Chrysanthemums

Abstract

Plant nodes were cultured on MS mediums supplemented with multiple concentrations of plant growth regulators. Sunny Snow produced the largest callus of 1.19×1.19 cm and obtained 93.33% of new shoots with 10 . 0 0 shoots per callus, and 0. 84 g callus fresh weight, when grew in MS medium supplemented with 1 mg/l BA + 0.1 mg/l IAA as compared to the control which no callus was produced. After 20 weeks of culturing, the second set showed that the control treatment of Sunny Snow gave the highest stem growth and the best canopy size of 9.00 and 5.60 cm respectively. Chompoo Phan gave the highest scores of chlorophyll content, i.e., chl a 652.14 µg/g FW, chl b 332.47 µg/g FW and carotenoid 165.92 µg/g FW. Besides, MDA appeared to be highest, 17.37 nmol/g FW when cultured on MS medium supplemented with 1 mg/l BA + 0.1 mg/l IAA.