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Laser-induced graphene electrochemical immunosensors for rapid and sensitive serological detection: A case study on dengue detection platform
Author(s)
Inlumphan, Supawee
Rattanawarinchai, Prapakorn
Leepheng, Piyawan
Luengrojanakul, Panuwat
Wuttikhun, Tuksadon
Obata, Michiko
Fujishige, Masatsugu
Takeuchi, Kenji
Uwanno, Teerayut
Horprathum, Mati
Porntheeraphat, Supanit
Sitthisuwannakul, Kannika
Phanthanawiboon, Supranee
Klamchuen, Annop
Date Issued
June 1, 2025
Type
Article
Abstract
Here, we present a diagnostic on a chip platform based on laser-induced graphene (LIG) electrochemical immunosensors for serological detection. The electrochemical immunosensors are fabricated through CO2 laser induction on polyimide (PI) sheets. Optimal electrochemical activity of LIG electrodes is obtained under optimized conditions of laser fluence. To verify the application, the serological detection platform was demonstrated. After functionalization with dengue virus (DENV) antigen, the LIG electrochemical immunosensors are able to sense the presence of mouse anti-flavivirus monoclonal (4G2) antibody in a wide linear working range of 25–20,000 ng/ml with the limit of detection (LOD) of 17.41 ng/ml. A specific recognition with 4G2 antibodies against with media protein and isotype is confirmed. Furthermore, the reliability of LIG electrochemical immunosensors compared to conventional enzyme-linked immunosorbent assay (ELISA) is verified through the NS1 antibodies identification in human blood serum clinical samples at room temperature. Our results highlight that the LIG-based electrode is a promising platform for electrochemical immunosensors, aimed at developing reliable and practical diagnostic tools for serological detection. These tools enable early diagnosis of infectious diseases, as well as non-invasive and rapid screening.
Citation
Sensors and Actuators Reports, 9, 2025