A new method to increase plant quantity through tissue culture on Vitex negundo L

Abstract

A new method to increase plant quantity through tissue culture on Vitex negundo L. was discovered. The influences of PGRs on shoot regeneration cultured on MS medium with 0.5, 1, 2, or 3 mg/L benzylaminopurine (BAP), meta-Topolin (mT) and Thidiazuron (TDZ) were resulted to be the greatest percentage of shoot development was 100% at 1 mg/L BAP in MS medium. The greatest average number of shoot multiplication was 4.40 shoots at 0.5 mg/L BAP in MS medium after 8 weeks of culture. For roots, the result of MS media fortified with 0.5, 1, 2, and 3 mg/L of indole 3 butyric acid (IBA) and naphthalene acetic acid (NAA) discovered that the excellent percentage of root production was 90% at 3 mg/L IBA. The highest average number of root production was 108.20 roots on 2 mg/L NAA after 8 weeks of culture. The plantlets were eventually transferred into the plastic pots. In this research work, the nodes were demonstrated to be an outstanding regenerating explant resource for Vitex negundo L. An effective regeneration strategy for plantlets derived from the nodes was demonstrated.