Establishment of condition and nanoparticle factors influencing plant regeneration from aromatic rice (Oryza sativa)

Abstract

Improvements on the callus induction and regeneration stages as well as the applications of nanoparticles using aromatic rice cv. KDML105 were investigated in this study. An efficient induction of plant regeneration from mature seeds was found in calli cultured under dark condition and was not transferred to the fresh medium (non-subculture) in each culture stage. Although, the size and biomass factors showed the better performance in calli cultured under light condition than dark but the regeneration frequency rate was lower than dark condition. Between the types and concentrations of nanoparticles applied for the regeneration enhancement. ZnO nanoparticles were found to be toxic, while TiO2 nanoparticles improved the plant regeneration. Optimum concentration (25 mg L-1) of TiO2 nanoparticles showed the high rate of green spots, plant regeneration and the ratio of the number of seedlings to the number of regenerated calli. Nonetheless, these factors may be valuable to establish an improvement in plant tissue culture system for propagation and/or genetic transformation in aromatic rice.