Glycomyces amatae sp. nov., isolated from a yellow-ringed grass moth (Amata sperbius)

Abstract

A novel mycelium-forming actinomycete strain, designated A-F 0318T, was isolated from a yellow-ringed grass moth (Amata sperbius) collected from Phitsanulok Province, Thailand. Long chains of non-motile cylindrical spores with a smooth surface developed on aerial mycelia. The polyphasic taxonomic study suggested that strain A-F 0318T belonged to the genus Glyco-myces. The 16S rRNA gene sequence analysis indicated that strain A-F 0318T was closely related to Glycomyces harbinensis LL-DO5139T with 97.94 % sequence similarity. The average nucleotide identity (ANI) based on blast, ANI based on the MUMmer algorithm and average amino acid identity values of strain A-F 0318T with G. harbinensis LL-DO5139T were 86.9, 89.1 and 84.24 %, respectively. The digital DNA–DNA hybridization value between A-F 0318T and its closest relative, G. harbinensis LL-DO5139T was 33.8 %. The digital G+C content of the genomic DNA was 71.7 mol%. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained ribose, xylose, glucose and galactose. The predominant menaquinone was MK-10(H4 ). The predominant fatty acids were iso-C16: 0, anteiso-C15: 0, anteiso-C17: 0 and iso-C16: 1 G. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, two unknown phosphoglycolipids and one unknown phospholipid. Based on comparative analysis of genotypic, phenotypic and chemotaxo-nomic data, the novel actinomycete strain A-F 0318T (=TBRC 13612T=NBRC 115417T) represents the type strain of a novel species, for which the name Glycomyces amatae sp. nov. is proposed.