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Molecular mechanism of antioxidant and anti-inflammatory effects of omega-3 fatty acids in perilla seed oil and rosmarinic acid rich fraction extracted from perilla seed meal on TNF-α induced A549 lung adenocarcinoma cells
Author(s)
Tantipaiboonwong, Payungsak
Chaiwangyen, Wittaya
Suttajit, Maitree
Kangwan, Napapan
Kaowinn, Sirichat
Khanaree, Chakkrit
Punfa, Wanisa
Pintha, Komsak
Date Issued
November 1, 2021
Type
Article
Abstract
Industrially, after the removal of oil from perilla seeds (PS) by screw-type compression, the large quantities of residual perilla seed meal (PSM) becomes non-valuable waste. Therefore, to increase the health value and price of PS and PSM, we focused on the biological effects of perilla seed oil (PSO) and rosmarinic acid-rich fraction (RA-RF) extracted from PSM for their role in preventing oxidative stress and inflammation caused by TNF-α exposure in an A549 lung adenocarcinoma culture model. The A549 cells were pretreated with PSO or RA-RF and followed by TNF-α treatment. We found that PSO and RA-RF were not toxic to TNF-α-induced A549 cells. Both extracts significantly decreased the generation of reactive oxygen species (ROS) in this cell line. The mRNA expression levels of IL-1β, IL-6, IL-8, TNF-α, and COX-2 were significantly decreased by the treatment of PSO and RA-RF. The Western blot indicated that the expression of MnSOD, FOXO1, and NF-κB and phosphorylation of JNK were also significantly diminished by PSO and RA-RF treatment. The results demonstrated that PSO and RA-RF act as antioxidants to scavenge TNF-α induced ROS levels, resulting in decreased the expression of MnSOD, FOXO1, NF-κB and JNK signaling pathway in a human lung cell culture exposed to TNF-α.
Citation
Molecules, 26(22), 2021