Selective electrochemical determination based on magnetic molecularly imprinted polymers for albumin detection

Abstract

Egg allergy is one of the most common allergies in humans. Allergenic egg protein as ovalbumin is the most abundant protein found in egg white. A selective electrode in electrochemical analysis for albumin from egg white was developed based on magnetic molecularly imprinted polymers (albumin-MMIPs) modified on a screen-printed electrode. Surface modification was applied using methyl methacrylate polymer to create surface specificity for albumin protein. The modified surface was characterized by a field-emission scanning electron microscope (FE-SEM). The average pore size was obtained by Brunauer-Emmett-Teller (BET) analysis, and the potentiostat obtained all electrochemical analysis. The albumin-MMIP electrode was selectivity with albumin, comparison with gluten, glycine, casein, and glucose. The modified electrodes present selectivity of 1.13 × 104 μAg-1ml and a limit of detection of 0.18 mg ml-1. The electrode can be applied to albumin protein detection in solution form in food industries.