Nanofibers derived from endophytic Chaetomium brasilense for growth stimulation of rice

Abstract

Chaetomiumn brasilense PT302 was endophytically isolated from rice var Supanburi 1 and Prathumthani 80 which firstly selected for investigation to promote the growth of rice. It is morphologically and molecular phylogenetic confirmation through ITS1 and ITS4 as Ch.brasilense PT302 compared to Achaetomium strumarium (JX863914) as out group. Crude metabolites (crude hexane, crude ethyl acetate and crude methanol) at 50 ppm from Ch. brasiliense were proved to promote seed germination of rice var. Supanburi 1 and Pathumtani 80 at 7 days which found significantly higher seed germination than the non-treated control. Nanofibers constructed Ch. brasiliense promoted significantly higher seed germination, plant height and number of tillers than the non-treated control in rice var Supanburi 1 and Prathumthani 80 in 7 days at low concentration of 5 ppm planted in Chachengsao and Bangkok soil series. Nanofibers derived from endophytic Ch. brasilense is actively promoted plant growth at lower concentration than crude metabolites. It is firstly reported the natural product nanofiber constructed from endophytic Ch. brasilense to stimulate plant growth.