Loading...
INVESTIGATION OF ANTIMICROBIAL CAPACITY AND ANTIOXIDANT EFFICIENCY FROM CHLORELLA SPP. CRUDE EXTRACTS
Date Issued
January 1, 2023
Type
Conference Paper
Abstract
Functional food, nutraceutical and pharmaceutical products are currently shared as an important part of the consumer market. Microalga plays a significant role in producing a source of good antioxidants including nutrient supplements such as chlorophyll, beta-carotene, astaxanthin etc. A Eukaryotic green microalga, Chlorella, is a unicellular microalga that itself is rich in a source of chlorophyll, carotenoids, the essential amino acids, bioactive compounds including fatty acids and these bioproducts make Chlorella as a great potential for human food and supplements, animal feed and biofuel feedstock. In this study, we aimed to test antimicrobial activity and antioxidant from four different Chlorella strains (Chlorella sp. KU11, Chlorella sp. V55, Chlorella sp. B2-59 and Chlorella sp. N11/59) for pharmaceutical and nutraceutical application. All four-ethanolic crude extracts of Chlorella were tested against six pathogens such as Streptococcus mutans ATCC25175, Bacillus subtilis ATCC6633, Staphylococcus aureus ATCC25923, Escherichia coli ATCC25922, Pseudomonas aeruginosa ATCC27853, and Candida albicans ATCC10231 using agar-well diffusion and the minimum inhibitory concentrating (MIC) assay. The result showed that 50 mg/mL of Chlorella sp. KU and Chlorella sp. V55 crude extracts could inhibit S. mutans, B. subtilis and S. aureus and 100 mg/mL of both crude extracts were able to inhibit E. coli, P. aeruginosa and C. albicans. From the minimum inhibitory concentrating (MIC) assay, we found that Chlorella sp. KU and Chlorella sp. V55 could inhibit S. mutans, B. subtilis, S. aureus E. coli, P. aeruginosa and C. albicans at 25, 50, 50, 100, 100 and 100 mg/mL, respectively and 50, 6.25, 25, 100, 100 and 100 mg/mL, respectively. The antioxidant activity of the four crude extracts were performed by DPPH radical scavenging assay and we found that the Chlorella sp. N11/59 crude extract showed the highest antioxidant efficiency with 63.73% and IC50 value was 2.8293 mg/mL. This finding suggested that microalgae can be a promising platform for pharmaceutical and nutraceutical application.
Citation
European Biomass Conference and Exhibition Proceedings, 1071-1075, 2023