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THE EFFECT OF SWEET POTATO PEEL HYDROLYSIS METHODS ON THE PRODUCTION OF BUTYRIC ACID AND BUTANOL USING CLOSTRIDIUM ACETOBUTYLICUM
Author(s)
Chiraporn, Pingmuang
Jurarat, Jumangmor
Doungkaew, Phiromphong
Noppol, Leksawasdi
Vorapat, Sanguanchaipaiwong
Date Issued
January 1, 2023
Type
Conference Paper
Abstract
Sweet potato (Ipomoea batatas L.) is a widely cultivated crop worldwide, yielding approximately 89.5 million tons annually. While sweet potatoes are consumed for various food purposes, there is a substantial amount of peel waste generated. Due to its high starch content, sweet potato peel has garnered interest as a potential feedstock for bioenergy conversion, particularly in bioalcohol production. However, this study reveals that the choice of hydrolysis method, either acidic or enzymatic, can significantly impact the products synthesized by Clostridium acetobutylicum. To conduct the experiments, sweet potato peel was milled into a powder and subjected to hydrolysis using 5% (v/v) H2SO4 at 90°C for 2 hours, or with 0.05% α-amylase (Termamyl®) at the same temperature for 2 hours, followed by 0.015% glucoamylase (AMG®) at 60°C for 4 hours. Each resulting hydrolysate, containing a reducing sugar concentration equivalent to 20 g/L, was then incorporated into a Glucose-Yeast extract-Casein-Cysteine (GYCC) medium for C. acetobutylicum cultivation. It was observed that the hydrolysis method employed significantly influenced various aspects of C. acetobutylicum growth, production time, and butyrate-to-butanol conversion. Notably, it is important to highlight that there was no significant difference (p>0.05) in the butanol concentration between the acidic hydrolysate (1.13 g/L) and the enzymatic hydrolysate (1.20 g/L). These findings emphasize the critical role of the hydrolysis method in determining the performance of C. acetobutylicum in terms of butanol production, growth, and conversion rates.
Citation
European Biomass Conference and Exhibition Proceedings, 1121-1124, 2023