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Effect of plant growth regulators on micropropagation of Vanilla aphylla and Vanilla planifolia sp. Variegate
Author(s)
Date Issued
December 1, 2018
Type
Article
Abstract
The plant growth regulators for propagating large number of vanillas in a short period of time was reported. Axillary buds were used from the explants of Vanilla aphylla and Vanilla planifolia sp. variegata that cultured on solid Gamborg's B5 medium supplemented with 0, 0.5, 1, 1.5 and 2 mg/L 6-benzylaminopurine (BA) for 8 weeks. The result showed that the highest average number of shoot proliferation and root induction were obtained at 1 mg/L BA of 3.2 cm. and 5.3 cm. respectively for Vanilla aphylla. Vanilla planifolia sp. variegata showed the highest shoot elongation of 1.9 cm. at 0.5 mg/L BA. Root induction was the highest of 0.9 cm. at 2 mg/L BA. After 12 weeks of culture The explants were grew the best after 12 weeks. They were transplanted into pots and covered by plastic bags for acclimatization and has maintained in greenhouse.
Citation
International Journal of Agricultural Technology, 14(7), 1357-1364, 2018