Loading...
Fungicidal activity of some essential oils and zinc oxide nanoparticles on Colletotrichum capsici and C. gloeosporioides causing chili anthracnose
Author(s)
Ahuja, A. J.
Date Issued
January 1, 2024
Type
Article
Abstract
Natural products are currently important sources in the antifungal spectrum properties for managing plant diseases. The study aimed to evaluate the potential protective role of essential oils (EOs) and zinc oxide nanoparticles (ZnO-NPs) against C. capsici (CC01) and C. gloeosporioides (CG02) causing chili anthracnose. The EOs extracted from 4 plants (clove bud, fennel sweet, kaffir lime, and holy basil) and ZnO-NPs with 25-50 nm in sizes revealed the fungicidal activity. All extracted essential oil with 5, 10, and 20 μL paper disc-1 (Ø 6 mm) and ZnO-NPs at 0, 500, 1,000, and 2,000 mg L-1 were tested for their effects on the mycelial growth of both fungi. The EOs inhibited the mycelial growth of pathogens and infection of chili fruits. The increasing volume of EOs increased the extent of fungal growth inhibition by the inverted petri-dish method. Clove bud oil at 20 μL paper disc-1 showed the maximum growth inhibition of CC01 and CG02 at 72.57 and 55.26%, respectively. It also had the greatest efficiency for controlling the lesions after inoculation with both fungal isolates on chili fruits. Moreover, ZnO-NPs with increasing concentrations could significantly inhibit the mycelial growth of both fungi. ZnO-NPs at 2,000 mg L-1 had the greatest effectiveness for inhibiting the mycelial growth of CC01 and CG02 at 48.54 and 53.76%, respectively. It also reduced the disease severity of chili fruits at 40.51 and 34.13% on CC01 and CG02, respectively. These results suggest that using EOs and ZnO-NPs could be a good and environmentally safe alternative for fungicides in controlling chili anthracnose.
Citation
Acta Horticulturae, 125-132, 2024