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Effect of subcritical solvent extraction conditions on amount of γ-oryzanol and γ-tocopherol in dawk pa-yom rice bran oil
Author(s)
Date Issued
January 1, 2021
Type
Article
Abstract
This research focused on the subcritical solvent extraction of phytochemicals γ-oryzanol and γ-tocopherol from upland rice bran (Dawk Pa-yom variety, DY). Subcritical solvent extraction was conducted using a batch reactor. The temperature was varied in range from 80 to 120°C for 20 to 60 min. The solvents in use were ethanol, methanol, and water. γ-Oryzanol and γ-tocopherol were simultaneously analyzed by HPLC. Antioxidant activity was determined by a DPPH radical assay. Methanol was able to extract a higher level of γ-tocopherol than ethanol and water. Extraction by methanol gave higher antioxidant activity than ethanol. Even though water could not extract γ-oryzanol, it was able to extract substances with higher yield of antioxidant activity than either methanol and ethanol. Increasing the extraction temperature increased the oil yield, γ-oryzanol and γ-tocopherol paralleled increase antioxidant activity. During the extraction process, some degradation of antioxidants was observed, and this showed the importance of kinetics parameters in this research.
Citation
Current Applied Science and Technology, 21(1), 151-161, 2021