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The Pigment Production from Monascus sp. U6V1, a Non-citrinin Producing Strain, in 5-L Stirred Tank Fermentor
Author(s)
Krairak, Nisa
Date Issued
May 1, 2022
Type
Article
Abstract
The significant maximum cell growth and pigment production of Monascus sp. U6V1 was observed in cassava starch peptone medium at 19.2±1.08 g/L and 39.48±2.42 UA500nm, respectively, by the cultivation at 200 rpm of shaking speed, 30oC for 7 days. Then, the experimentation was carried in 5-L stirred tank fermentor for the optimal agitation speed and aeration rate. At 500 rpm of agitation speed and 1.00 VVM of aeration rate, Monascus sp. U6V1 gave significant pigment production, pigment yield and specific pigment production rate at 40.17±1.45 UA500nm, 0.686 UA500nm/g and 0.143 UA500nm/g/h, respectively. When the cultivation was observed for the optimal aeration rate. The aeration rate at 0. 75 VVM and 1. 00 VVM gave the insignificant pigment concentration and specific pigment production rate at 36. 04±1. 04 and 40. 17±1. 45 UA500nm and 0. 145 and 0.143 UA500nm/g/h, respectively. The shear rate and oxygen shortage during exponential phase showed some effects on pigment production in stationary phase. At the end of cultivation, all samples were analyzed for the citrinin content. The result showed that citrinin content was not detected.
Citation
Burapha Science Journal, 27(2), 1135-1152, 2022
