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Gene Sequence Variations of Carotenoid Biosynthesis Pathway in Thai Rice Varieties (Oryza sativa L.) from Transcriptome and Whole Genome Sequencing Analysis
Author(s)
Janphen, Kanokwan
Sangtong, Varaporn
Sakulsingharoj, Chotipa
Leethanatudom, Suparat
Poeaim, Anurug
Pongjaroenkit, Saengtong
Date Issued
May 1, 2022
Type
Article
Abstract
Lutein is a product from carotenoid biosynthetic pathway which is highly found in black rice pericarp. The purpose of this study was to identify sequence variations of genes in carotenoid biosynthetic pathway from Thai rice varieties with white (no lutein) and black (high lutein) pericarp by transcriptome and genome analyses. The sequences of 12 genes in the carotenoid synthesis showing variations between white and black rice were detected. The variations in gene regions were found in 5 genes which were phytoene desaturase (PDS), zeta-carotene isomerase (ZISO), lycopene epsilon-cyclase (LCYe), cytochrome P450 carotene beta-hydroxylase (Lut5, CYP97A) and cytochrome P450 type B (CYP97B). High levels of sequence variations in upstream region, gene region and downstream region were found in ZISO, CYP97A and CYP97B genes. Therefore, these three genes were probably key candidate genes related to lutein biosynthesis. The sequence variations of these genes in the carotenoid biosynthesis pathway will be used to develop DNA markers for selection of high lutein accumulation in rice breeding program.
Citation
Burapha Science Journal, 27(2), 900-920, 2022