The Evaluation of a Lateral Flow Strip Based on the Covalently Fixed “End-On” Orientation of an Antibody for Listeria monocytogenes Detection

Thongmee, Patsara; Ngernpimai, Sawinee; Srichaiyapol, Oranee; Mongmonsin, Urairat; Teerasong, Saowapak; Charoensri, Nicha; Wongwattanakul, Molin; Lulitanond, Aroonlug; Kuwatjanakul, Waewta; Wonglakorn, Lumyai; Kendal, Rinjong Promsorn; Chompoosor, Apiwat; Daduang, Jureerut; Tippayawat, Patcharaporn

doi:10.1021/acs.analchem.4c00533

The Evaluation of a Lateral Flow Strip Based on the Covalently Fixed “End-On” Orientation of an Antibody for Listeria monocytogenes Detection

Author(s)

Thongmee, Patsara

Ngernpimai, Sawinee

Srichaiyapol, Oranee

Mongmonsin, Urairat

Teerasong, Saowapak

Charoensri, Nicha

Wongwattanakul, Molin

Lulitanond, Aroonlug

Kuwatjanakul, Waewta

Wonglakorn, Lumyai

Kendal, Rinjong Promsorn

Chompoosor, Apiwat

Daduang, Jureerut

Tippayawat, Patcharaporn

Date Issued

May 28, 2024

Type

Article

DOI

10.1021/acs.analchem.4c00533

Abstract

In this study, the covalently fixed “end-on” orientation of a monoclonal Listeria monocytogenes antibody (mAb-Lis) to amino terminated oligo (ethylene glycol)-capped gold nanoparticles (NH2-TEG-AuNPs) was used to fabricate an in-house lateral flow strip (LFS), namely, the fixed “end-on” Lis-mAb-NH-TEG-AuNPs LFS. The aim was to evaluate the performance of the fixed “end-on” Lis-mAb-NH-TEG-AuNPs LFS in detecting L. monocytogenes. The proposed LFS enabled the sensitive detection of L. monocytogenes in 15 min with a visual limit of detection of 10² CFU/mL. Quantitative analysis indicated an LOD at 10 CFU/mL. The fixed “end-on” Lis-mAb-NH-TEG-AuNPs LFS showed no cross-reactivity with other pathogenic bacteria and practical performance across different food matrices, including human blood, milk, and mushroom samples. Furthermore, the clinical performance of the fixed “end-on” Lis-mAb-NH-TEG-AuNPs LFS for detecting L. monocytogenes was evaluated by using 12 clinical samples validated by the hemoculture method. It demonstrated excellent concordance with the reference methods, with no false-positive or false-negative results observed. Therefore, the fixed “end-on” Lis-mAb-NH-TEG-AuNPs LFS serves as a promising candidate for a point-of-care test (POCT), enabling the rapid, precise, and highly sensitive detection of L. monocytogenes in clinical samples and contaminated food.

Citation

Analytical Chemistry, 96(21), 8543-8551, 2024