Charoenying, Patchanee
Loading...
Preferred name
Charoenying, Patchanee
Alternative Name
Charoenying, P.
Main Affiliation
Email
patchanee.ch@kmitl.ac.th
23 results
Now showing 1 - 10 of 23
- Some of the metrics are blocked by yourconsent settings
Item type:Publication, Effects of natural herbicide from tagetes erecta on echinochloa crus-galli (L.) beauv(2018-01-01); ; We studied the inhibitory effects of leaves extracts of Tagetes erecta on weed-seed germination and the mechanisms involved. Hydroethanolic crude extract (OR) from Tagetes erecta dried leaves was partitioned into four fractions: hydrolyzed (HY), aqueous (AQ), neutral (NE) and acidic (AE). The inhibitory effects of crude extracts and its fractions were evaluated on germination and seedlings growth of Echinochloa crus-galli (L.) Beauv. The total phenolic and flavonoid contents of each fraction were also determined. Germination and seedling growth were drastically inhibited by HY and AE followed by NE, OR and AQ. The HY and AE fractions had higher concentrations of both phenolics and flavonoids than other fractions. The HY fraction was selected as most potent for development as natural herbicide due to its high yield and strong inhibitory effects. The HY fraction was formulated into a soluble concentrate product (SCT) and its inhibition potential and modes of action were investigated. The SCT drastically inhibited the seed germination of E. crus-galli, inhibiting both imbibition, α-amylase activity and also seedling growth. Cytogenetic bioassays of SCT on root-tip cells of Allium cepa L. showed that it inhibited the mitosis and thereby the cell division. It is likely that a natural herbicide may be developed from the hydrolyzed fraction of a hydroethanolic extract of Mexican marigold leaves. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Herbicidal activities of some allelochemicals and their synergistic behaviors toward amaranthus tricolor L.(2017-11-01); ; Seven allelochemicals, namely R-(+)-limonene (A), vanillin (B), xanthoxyline (C), vanillic acid (D), linoleic acid (E), methyl linoleate (F), and (±)-odorine (G), were investigated for their herbicidal activities on Chinese amaranth (Amaranthus tricolor L.). At 400 μM, xanthoxyline (C) showed the greatest inhibitory activity on seed germination and seedling growth of the tested plant. Both vanillic acid (D) and (±)-odorine (G) inhibited shoot growth, however, apart from xanthoxyline (C), only vanillic acid (D) could inhibit root growth. Interestingly, R-(+)-limonene (A) lightly promoted root length. Other substances had no allelopathic effect on seed germination and seedling growth of the tested plant. To better understand and optimize the inhibitory effects of these natural herbicides, 21 samples of binary mixtures of these seven compounds were tested at 400 μM using 0.25% (v/v) Tween(r) 80 as a control treatment. The results showed that binary mixtures of R-(+)-limonene:xanthoxyline (A:C), vanillin:xanthoxyline (B:C), and xanthoxyline:linoleic acid (C:E) exhibited strong allelopathic activities on germination and seedling growth of the tested plant, and the level of inhibition was close to the effect of xanthoxyline (C) at 400 μM and was better than the effect of xanthoxyline (C) at 200 μM. The inhibition was hypothesized to be from a synergistic interaction of each pair of alleochemicals. Mole ratios of each pair of allelochemicals ((A:C), (B:C), and (C:E)) were then evaluated, and the best ratios of the binary mixtures A:C, B:C and C:E were found to be 2:8, 2:8, and 4:6 respectively. These binary mixtures significantly inhibited germination and shoot and root growth of Chinese amaranth at low concentrations. The results reported here highlight a synergistic behavior of some allelochemicals which could be applied in the development of potential herbicides. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Antioxidant and Antibacterial Activities of Jasminum officinale L. f. var. grandiflorum (L.) Kob. Leaf Extracts(2024-05-01); ; The investigation of phytochemicals and their effects on plants is currently receiving significant attention due to the well-known fact that plants contain secondary metabolites that have high levels of usefulness in various applications. These applications include food additives, biopesticides, and pharmaceuticals. In a recent study, we thoroughly examined the total phenolic content (TPC), and the antioxidant, and antibacterial properties of Jasminum officinale L.f. var. grandiflorum (L.) Kob. (also known as Spanish jasmine) leaf extracts, providing valuable insights into its potential benefits. Our study performed a crude methanol (ME) extraction of Spanish jasmine leaves with acid-base solvent partitioning, resulting in an acidic fraction (AE), a neutral fraction (NE), and an aqueous fraction (AQ). The Folin-Ciocalteu method was used to determine the TPC of the extracts. The AE fraction displayed the highest TPC (79.8±0.61 mgGAE/g dry weight), followed by the NE and AQ fractions. The extracts were further evaluated for their antioxidant activity using the DPPH assay. The AE fraction had the highest antioxidant activity with an EC<inf>50</inf> value of 4.6±0.40 µg/mL. The main active compound, oleuropein, was isolated and determined using spectral data. The isolated compound displayed excellent antioxidant activity with an EC<inf>50</inf> value of 13.0±0.06 µg/mL. Moreover, the antibacterial activity of the Spanish jasmine leaf extracts was calculated using the disc diffusion method based on the minimum inhibitory concentration. The AE fraction exhibited activity against the Gram-negative bacteria, Vibrio parahaemolyticus, with a MIC value of 0.78 µg/mL. The AE fraction also displayed the highest degree of activity against Staphylococcus strains, including drug-resistant strains (MRSA and VRSA), with a MIC range of 0.39-12.5 µg/mL. It is important to highlight that the AE fraction demonstrated the highest level of activity against all VRSA strains tested despite their resistance to vancomycin and oxacillin. These findings strongly indicate that Spanish jasmine leaves possess potent natural antioxidant and antibacterial properties, positioning them as a highly promising plant source. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, An allelopathic substance isolated from Zanthoxylum limonella Alston fruit(2010-06-28); ; Bioassay-guided fractionation of the ethyl acetate extract of the Zanthoxylum limonella Alston fruits led to the isolation of a phenolic compound named xanthoxyline. The allelopathic effects of xanthoxyline were evaluated on Chinese amaranth (Amaranthus tricolor L.) and Barnyardgrass (Echinochloa crus-galli (L.) Beauv.) under laboratory conditions. At a concentration of 2500 μM, xanthoxyline completely inhibited seed germination and growth of Chinese amaranth, while this compound showed a significantly inhibitory effect on seed germination of Barnyardgrass by 43.59% and inhibited shoot and root length by 71.56% and 87.48%, respectively. To determine the mechanism through which xanthoxyline exerts its toxicity, the cytogenetic bioassay was conducted. Roots of Allium cepa L. (onion) were treated with a series of concentrations, ranging from 15.625 to 125 μM for 18 h. Cytogenetic studies of onion roots showed a decrease of the mitotic index in the exposed roots dependent on the concentration of xanthoxyline. Likewise, the mitotic phase index was altered in onion incubated with xanthoxyline. Analyses of the abbreviation types showed spindle disturbances at late prophase, sticky metaphase, c-mitosis, non-oriented chromosome at metaphase, sticky anaphase, delayed anaphase and diagonal at telophase for the onion roots exposed to xanthoxyline. © 2010 Elsevier B.V. All rights reserved.1 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Physiological and cellular mechanisms of natural herbicide resource from Aglaia odorata Lour. On bioassay plants(2012-07-01); ; A bioassay-guided fractionation of the allelochemicals of the ethyl acetate fraction extracted from Aglaia odorata led to isolation of a bisamide, odorine. The growth inhibitory effects of odorine and ethyl acetate fraction were studied for comparison on Echinochloa crus-galli. Odorine and ethyl acetate fraction of A. odorata could inhibit the germination and seedling growth of E. crus-galli, with ethyl acetate fraction being more potent. Thus, ethyl acetate fraction was selected for further experiment, E. crus-galli seeds were studied the effects of the ethyl acetate fraction from A. odorata leaves in wet-table powder formulation on imbibition and α-amylase activity. It was found that treated seeds showed lower imbibition and α-amylase activity. The results of cytogenetic bioassay in Allium cepa roots showed that ethyl acetate fraction inhibited cell mitosis and induced mitotic abnormalities resulting from its action on chromatin organization and mitotic spindle in the exposed roots.1 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Herbicidal Activity of Flavokawains and Related trans-Chalcones against Amaranthus tricolor L. And Echinochloa crus-galli (L.) Beauv.(2019-12-10); ; Flavokawains have a broad spectrum of biological activities; however, the herbicidal activity of these naturally occurring chalcones has been less investigated. Flavokawains and their analogues were prepared by the Claisen-Schmidt condensation reaction between xanthoxyline (or aromatic ketones) and a variety of aromatic and heteroaromatic aldehydes. These compounds were then evaluated for their inhibitory effect against representative dicot and monocot plants. Among 45 synthetic chalcones, derivatives containing phenoxyacetic acid, 4-(N,N-dimethylamino)phenyl, N-methylpyrrole, or thiophenyl groups inhibited the germination and growth of Chinese amaranth (Amaranthus tricolor L.) with moderate to high degrees compared to commercial butachlor. For barnyardgrass (Echinochloa crus-galli (L.) Beauv.), most of the thiophenyl chalcones interrupted shoot and root emergence. This finding highlighted the importance of functional groups on the herbicidal activity of chalcones. The level of inhibition also depended on the applied concentrations, plant species, and plant organs. (E)-2-(2-(3-Oxo-3-(thiophen-2-yl)prop-1-enyl)phenoxy)acetic acid (14f) was the most active compound among 45 derivatives. This chalcone could be a promising structure for controlling the germination and growth of weeds. The structure-activity relationship results provide useful information about the development of active chalconoids as novel natural product-like herbicides.1 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Bioassay-Guided Extraction and Isolation of Natural Herbicides from Dried Zanthoxylum limonella Alston Fruits(2025-01-01); ; ; ; Jongkon, NathjananWeeds are problematic plant species around the world. Various strategies exist for controlling weeds, but chemical treatment remains the preferred method, particularly when using natural substances. In this research, a crude aqueous-methanol extract from dried Zanthoxylum limonella fruits was acid-base partitioned into four fractions: neutral extract (NE), acid extract (AE), basic extract (BE), and aqueous extract (AQ). These fractions were further separated into seventeen subfractions: NEF1 to NEF7, AEF1 to AEF5, and BEF1 to BEF5, which were then tested for herbicidal activity against the growth of Chinese amaranth (Amaranthus tricolor) and barnyard grass (Echinochloa crus-galli). Active subfractions were isolated via column chromatography and identified using spectroscopic methods, yielding seven active compounds: xanthoxyline (1), tambulin (2), atanine (3), prudomestin (4), skimmianine (5), p-methoxybenzoic acid (6), and methyl caffeate (7). Compounds 2–7 had not been previously reported in Z. limonella. Xanthoxyline (1) was identified as the most potent botanical herbicide, fully inhibiting seed germination of Chinese amaranth and barnyard grass. This compound also decreased seed imbibition and α-amylase activity in both species. Molecular docking studies on the α-amylase enzyme (PDB ID: 1BG9) revealed that the aromatic, hydroxy, and carbonyl groups of xanthoxyline (1) interact with the enzyme's active sites.8 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Evaluation of allelopathic, decomposition and cytogenetic activities of Jasminum officinale L. f. var. grandiflorum (L.) Kob. on bioassay plants(2010-07-01); ; Methanolic extracts prepared from dried leaves of Jasminum officinale f. var. grandiflorum (L.) Kob. (Spanish jasmine) inhibited seed germination and stunted both root and shoot length of the weeds Echinochloa crus-galli (L.) Beauv. and Phaseolus lathyroides L. The main active compound was isolated and determined by spectral data as a secoiridoid glucoside named oleuropein. In addition, a decrease in allelopathic efficacy appeared as the decomposition periods increased. The mitotic index in treated onion root tips decreased with increasing concentrations of the extracts and longer periods of treatment. Likewise, the mitotic phase index was altered in onion incubated with crude extract. Furthermore, crude extract produced mitotic abnormalities resulting from its action on chromatin organization and mitotic spindle. © 2010 Elsevier Ltd. All rights reserved.2 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Phytotoxic effects of essential oil from Cymbopogon citratus and its physiological mechanisms on barnyardgrass (Echinochloa crus-galli)(2013-01-01); Lemongrass (Cymbopogon citratus) essential oil was identified by GC-MS for their major compound constituents; six compounds representing 92.59% of the total essential oil were identified. The main constituent was citral (76.00%). In a laboratory bioassay, high concentration of essential oil significantly inhibited germination and seedling growth of Echinochloa crus-galli and affecting α-amylase activity of seeds. In a glass house bioassay, essential oil at concentrations of 1.25, 2.5, 5 and 10% (v/v) was foliar-applied on E. crus-galli at 28 days after sowing at spray volume of 1000Lha <sup>-1</sup>. Observation of leaf wilt symptoms was noted at 6h after treatment. Chlorophyll a, b and carotenoid content was decreased with concentrations of essential oil, indicating that essential oil interferes with photosynthetic metabolism. C. citratus essential oil caused an electrolyte leakage indicating membrane disruption and loss of integrity. Treated leaves exhibited an increase in thiobarbituric acid reactive substances (TBARS), suggesting lipid peroxidation. It is to conclude that essential oil is phytotoxic and could be utilized as a natural herbicide for future weed control. © 2012 Elsevier B.V.1 - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Synthesis and Cytotoxicity Evaluation of Novel C-3 Aminocarbamate Pregnenolone Derivatives(2025-03-06); Cancer is one of the leading causes of death worldwide. There are many ongoing studies in the search for new treatments or drugs to combat cancer. Similarly, in this research, twelve 3-aminocarbamate pregnenolones (2a–2l) were designed, synthesized, and evaluated for their cytotoxicity against five cancer cell lines: Human hepatocellular carcinoma (HepG2), Human colon adenocarcinoma (HT-29), Human oral cavity carcinoma (KB), Human breast adenocarcinoma (MCF-7), Murine leukemia (P388), and one normal cell line, African green monkey kidney fibroblast (Vero), using the MTT assay. Notably, 3-aminobenzylcarbamate pregnenolone (2b), 3-diaminoheptylcarbamate pregnenolone (2f), and 3-diaminopropanolcarbamate derivative (2i) were the most potent against these cancer cell lines. Specifically, for P388 cell lines, these compounds were more potent than the positive control drug, vinblastine sulfate salt. Results from the SAR study demonstrated that the length of the alkyl chain of diaminocarbamate derivatives was crucial for their anticancer properties. These findings will be useful in the future research and development of anticancer drugs.1