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    Modified atmosphere for thrip disinsection on cut lotus flowers
    (2018-01-01)
    Bumroongsook, S.
    ;
    In Thailand, lotus flowers are one of exportable products for flower trade industry. Common blossom thrip (Frankliniella schultzei) contamination on cut lotus flowers after harvest has been a major problem for export. F. schultzei is a quarantine insect pest. Modified atmospheres (MA) without oxygen content are considered an alternative to methyl bromide fumigation to control thrips. MA treatment has been used to control insect pests in agricultural product commodities. Different combinations of carbon dioxide, nitrogen, ozone levels, and treatment times were used to effect mortality of the common blossom thrips and postharvest quality of cut lotus flowers. Exposure to different concentrations of O<inf>3</inf> fumigation for 2 days could kill almost all F. schultzei (100% of larvae and ≥ 96% of adults). However, a concentration of 50-250 ppm of O<inf>3</inf> could not control F. schultzei on lotus flowers completely. O<inf>3</inf> fumigation caused color change in lotus flowers. High concentrations of O<inf>3</inf> (≥ 150 ppm) had a negative effect on the visual quality of lotus flowers. The results revealed that thrips mortality increased with increased CO<inf>2</inf> level and storage time. 100% CO<inf>2</inf> caused 100% mortality of both adults and larvae of F. schultzei when there was a 6-h exposure. MA were more effective in disinfecting of thrips on cut lotus flowers after 9 h fumigation to ≥ 50% CO<inf>2</inf> caused complete mortality to F. schultzei. There was not much difference in lotus color in response to atmosphere modified by a combination of CO<inf>2</inf> and N<inf>2</inf>. Therefore, CO<inf>2</inf> disinfestation treatment has the potential to be developed commercially as an alternative postharvest control for common blossom thrips on lotus flowers.
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    Life History Characteristic and Larval-Pupal Parasitoids of the Dolichandrone Weevil, Cionus sp. (Coleoptera:Curculionidae)
    (2019-01-01) ;
    Tigwattananont, Saen
    ;
    Bumroongsook, Suvarin
    Mangrove trumpet trees (Dolichandrone serrulata (Wall. ex DC.) Seem are popular in Thailand and used as edible plants and ornamental trees. Cionus sp.(Dolichandrone weevil) feeds on Mangrove trumpet trees but data on its life history is very limited. Based on field collection from the Mangrove trumpet tree from public parks in Nonthaburi province, Thailand and laboratory studies, it provides the morphological description of larvae and adults, new data on growth and development of Cionus sp. as well as its larval-pupal parasitoids. The insect species has four different life stages: egg, larva, pupa and adult. After adult emergence for 5-7 days, mating will occur during daytime and the copulation lasts for 5-10 hours. The female laid eggs in a group of 3-7 eggs. A minimum of 14.71 days is required for passing through the egg, larval and pupal stages. Females live slightly longer than males (82.35 and 81.20 days, respectively). Upon the completion of larval development, it will build a cocoon, pupate in there and attach to the ventral or dorsal part of leaf surface. The adults normally emerge in the morning. It exibits death-feigning behaveior when being disturbed. In nature, both adults and larvae are commonly found in the rainy season. These insect larvae feed on leaves and young twig surface of Mangrove trumpet trees whereas adults prefer young leaves. Heavy infestation can stunt the tree’s growth and cause dieback. The larvae and pupae were observed parasitized by the hymenopteran parasitoid, Entedon sp. The parasitization occurrence during May, 2017 to July, 2018 was 6.25-24.19% in larvae and 0.00-62.50 % in papa. Further studies should be conducted to evaluate the efficacy of Entedon sp. as the potential biological control agent for Cionus sp population reduction in home gardens and residential areas.
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    Toxicity of synthetic insecticides on Tetragonula pagdeni (Hymenoptera Apidae)
    (2024-01-01) ;
    Chaisuekul, Chatchawan
    Stingless bees are important pollinators in the ecosystem and crop production in most subtropical and tropical regions of the world. However, the global decline of bee populations is a serious concern. Insecticides are considered crucial factors affecting bee survival. In this study, the acute toxicity of selected synthetic insecticides (imidacloprid, profenofos, and lambda-cyhalothrin) on a stingless bee, Tetragonula pagdeni (Schwarz) (Hymenoptera Apidae), was investigated and the LD50 and LC50 determined through topical, oral, and contact exposure under laboratory conditions. The estimated topical LD50 values for imidacloprid, lambda-cyhalothrin, and profenofos were 0.06, 0.53, and 45.39 ng active ingredient (a.i.)/bee, respectively. The corresponding estimated LD50 values from oral bioassays were 0.66, 7.52, and 75.04 ng a.i./bee, and LC50 values for contact exposure were 48.74, 4,339.83, and 3,062.81 ng a.i./cm<sup>2</sup>. Overall, imidacloprid exhibited the highest toxicity among the evaluated insecticides for all routes of exposure. In addition, insecticide toxicity was higher in the topical bioassay than in oral and contact bioassays. These findings demonstrate that this stingless bee is extremely vulnerable to insecticide use. Therefore, selection of insecticides in pest control programs should consider mitigation of the risk to and detrimental effects on stingless bees.
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    Consumption efficiency of Wolf and lynx spiders, pardosa pseudoannulata and oxyopes javanus, on insect pests of asiatic pennywort
    (2018-07-01)
    Bumroongsook, S.
    ;
    Name, J.
    ;
    Asiatic pennywort is a plant common to Central and Southeast Asia and is used for folk medicine. The most common predatory spiders found in the Asiatic pennywort growing areas were Wolf and lynx spiders, Pardosa pseudoannulata Bösenberg and Strand and Oxyopes javanus Thorell. They were observed the onsuming two important insect pests of pennywort: Young pennywort cutworm, Zonoplusia ochreata Walker, 1865 and a leafhopper, Empoascanara alami Ahmed. The consumption efficiency associated with spider sex and starvation condition of these two predatory spiders on the leafhopper and pennywort cutworm was assessed in the laboratory. The results showed that female spiders consumed more leafhoppers and pennywort cutworms than did the males. Both predatory spiders preferred the second larval instar of pennywort cutworm. The female and male of O. javanus consumed 18.07 and 17.11 insects/day, respectively, and P. pseudoannulata 14.39 and 12.18 insects/day, respectively. Starvation for a prolonged food of time did not increase hunger level of the spiders. The use of these two spiders combined, in comparison with O. javanus alone, against leafhoppers was not significant (P>0.05). However, P. pseudoannulata and O. javanus are important components of the natural enemy complex in pennywort and therefore they should be conserved to aid insect control in pennywort.
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    Toxicity for Control of Frankliniella schultzei and Selenothrips rubrocinctus (Thysanoptera: Thripidae) of Several Common Synthetic Insecticides
    (2022-06-01)
    Thrips are serious pests of several kinds of crop plants throughout the world. Their attack leads to loss in plant production. Common blossom thrips, Frankliniella schultzei (Trybom), and red-banded thrips, Selenothrips rubrocinctus (Giard) (both Thysanoptera: Thripidae), are serious insect pests attacking various plants, such as tomato (Solanum esculentum Miller [Solanaceae]), mango (Mangifera indica Linnaeus [Anacardiaceae]), avocado (Persea americana Miller [Lauraceae]), and lotus flower (Nelumbo nucifera Gaertn. [Nelumbonaceae]). Currently, basic thrips control relies on synthetic insecticides. However, the toxicity of each existing insecticide for control of some specific pest species still has not been well established. This study evaluated and reported the toxicity of 6 insecticides (chlorantraniliprole, cypermethrin, carbosulfan, fipronil, abamectin, and spinetoram) for control of the 2 thrips species mentioned above. The evaluation was done by exposing 10 thrips to a bean dipped in insecticide, and mortality was recorded after 24 h of exposure under laboratory conditions. Spinetoram was the most toxic to F. schultzei with an estimated LC50 of 0.05 ng per μL. For red-banded thrips, S. rubrocinctus, the most toxic insecticides were abamectin, spinetoram, and fipronil, with LC50 values of 1.67, 1.85, and 4.23 ng per μL, respectively. On the other hand, the least toxic insecticide to common blossom thrips and red-banded thrips was chlorantraniliprole, with LC50 values of 270.51 and 641.08 ng per μL, respectively. Overall, among the tested chemicals, spinetoram was the most effective in controlling these pests. The findings from this study will benefit developers of insecticide management strategies in thrips control programs.
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    Bionomics of the australian hawk moth, theretra laterillii lucasii (Walker) (Lepidoptera: Sphingidae)
    (2018-07-01) ;
    Tigvattananont, S.
    Theretra latreillii lucasii (Walker) is an important insect pest of water primrose, Ludwigia hyssopifolia (G. Don). Eggs and larvae of T. l. lucasii were collected from L. hyssopifolia and raiseed in the entomological laboratory, King Mongkut's Institute of Technology Ladkrabang. The number of laid eggs was 200-240 eggs per adult female. The egg incubation period lasted 3.47±0.15 days. After egg hatching, T. l. lucasii larvae go through five instars of developmental stages. Duration time of 1st to 5th instars was 3.79±0.50, 4.27±0.57, 3.26±0.50, 3.06±0.32 and 6.59±0.61 days, respectively. The body length was 4.73±1.16, 11.2±1.08, 16.33±1.35, 27.13±1.96 and 53.27±9.19 mm for 1st to 5th instar, respectively. The head capsule for 1st to 5th instar was 0.59±0.08, 0.91±0.06, 1.10±0.030, 2.28±0.15 and 3.87±0.18 mm wide, respectively. The length in mm for dorsal horn of 1st to 5th instar was 2.13±0.72, 3.67±0.62, 5.13±0.58, 5.57±0.46 and 5.63±0.64 mm long, respectively. Adults are medium in sizes with prominent heads and big eyes. The female lived longer than the male (8.40±0.92 and 6.47±0.91days, respectively). The principal host plants of T. l. lucasii are L. hyssopifolia and L. octovalvis in family Onagraceae. Moreover, Cayratia trifolia in family Vitaceae were found to be the food plant for T. l. lucasii.