Sanguanchaipaiwong, Vorapat

Preferred name

Alternative Name

Sanguanchaipaiwong, V.

Main Affiliation

School of Science

vorapat.sa@kmitl.ac.th

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Enzymatic hydrolysis of cassava stems for butanol production of isolated Clostridium sp.
(2020-02-01)
Saekhow, B.
;
Chookamlang, S.
;
Na-u-dom, A.
;
Leksawasdi, N.
;
Sanguanchaipaiwong, V.
This research focused on the hydrolysis of cassava stems (CS) and subsequent utilization as a carbon source for the cultivation of isolated Clostridium sp. To yield the highest amount of reducing sugars (RS), the studies on the pretreatment with sodium hydroxide (NaOH) and the hydrolysis with cellulases, amylases, and mixed enzymes were carried out. Afterwards, the hydrolysate was utilized for the cultivation of isolated Clostridium sp. Experimental results revealed that CS after 1.0 M NaOH pretreatment at 121 °C for 15 min and cellulase hydrolysis (Accellerase® 1500, 2500 CMC U/g CS) obtained 10.94 ± 0.29 g/L RS concentration. Hydrolysis of CS with amylases (Termamyl® 120, 1.2 U/g CS and AMG 300L™ 3.5 U/g CS) provided 34.85 ± 0.75 g/L RS and the maximum RS amount of 47.90 ± 0.39 g/L was obtained from the hydrolysis with mixed enzymes (Termamyl® 120, 1.2 U/g CS, AMG 300L™ 3.5 U/g CS followed by Accellerase® 1500, 2500 CMC U/g CS). From the cultivation of Clostridium sp. G10 using CS hydrolysate, the highest dry cell weight concentration of 1.28 ± 0.07 g/L was obtained with 11.68 ± 0.31 g/L butanol. It could be concluded that CS hydrolysate was comparable with glucose for utilization as a carbon source for butanol production.
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Using Glycerol as a Sole Carbon Source for Clostridium beijerinckii Fermentation
(2017-01-01)
Sanguanchaipaiwong, Vorapat
;
Leksawasdi, Noppol
Butanol is a promising biofuel with its fuel properties which are similar to gasoline and able to be synthesized from acetone-butanol-ethanol fermentation of Clostridium sp. This study has been focused on using glycerol, a by-product from biodiesel manufacturing as a carbon source for Clostridium beijerinckii TISTR 1390. The culture was cultivated at 37°C in P2 medium with 20-60 g/L glycerol under anaerobic condition, compared with glucose as a control. Samples were collected periodically for the analysis of viable cell concentration, reducing sugar and metabolite concentrations. It has been found that C. beijerinckii grown much slower in glycerol. After 168 h cultivation, the cell growth in 20 g/L glycerol has achieved the maximum concentration of 4.78 × 106 CFU/mL. In P2 medium containing glucose, the amount of viable cell (3.93 x 106 CFU/mL with 20 g/L glucose) had reached the maximum level at 24 h. The much longer period for highest cell number might be the reason that C. beijerinckii could not produce butanol from merely glycerol. On the other hand, the butanol concentration of 8.12 g/L was obtained from 60 g/L glucose. Glycerol probably induced C. beijerinckii in switching to a reductive pathway.
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Butanol production by Clostridium beijerinckii from pineapple waste juice
(2018-01-01)
Sanguanchaipaiwong, Vorapat
;
Leksawasdi, Noppol
Renewable energy has received increasing attention, due to global energy crisis and limited supply of fossil fuels. Butanol is one of the alternative biofuels with the similar energy properties as gasoline, such as, energy density and heat of vaporization. It could be produced by Clostridia via acetone-butanol-ethanol fermentation from various renewable sources. To obtain economical raw materials, pineapple waste juice was utilized as a carbon source for Clostridium beijerinckii TISTR 1461. The juice was collected from ‘Pattavia' pineapple waste and utilized to prepare culture medium. The maximum viable C. beijerinckii concentration (2.40 ± 0.12 x 108 CFU/mL) was obtained at 168 h of cultivation with pineapple waste juice under anaerobic condition at 37 °C. The butanol concentration of 3.14 ± 0.16 g/L was subsequently produced. A yield of 0.08 g butanol∙g-1 reducing sugars was achieved suggesting the necessity to improve fermentation process for higher level of butanol concentration.
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Kinetic parameters of Candida tropicalis TISTR 5306 for ethanol production process using an optimal enzymatic digestion strategy of assorted grade longan solid waste powder
(2019-11-01)
Wattanapanom, Saengkae
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Muenseema, Jidapa
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Techapun, Charin
;
Jantanasakulwong, Kittisak
;
Sanguanchaipaiwong, Vorapat
The optimal enzymatic digestion strategy for assorted grade whole fruit longan solid waste powder (WF–LSWP) to release the highest level of fermentable sugars for subsequent production of ethanol using Candida tropicalis TISTR 5306 has been reported for the first time in this study with several important fermentation kinetic parameters. WF–LSWP contained relatively low lignin content (5.79 ± 0.43 %(w/w)) with the presence of relatively high starch and pectin contents of 27.9 ± 0.86% (w/w) and 2.07 ± 0.16% (w/w), respectively. Pretreatment by alkali and saturated steam before enzymatic digestion step did not result in the improvement of overall sugars being released. The implementation of commercial enzyme mixture (amylase, glucoamylase, cellulase, and xylanase) for one step enzymatic digestion at 50°C for 48 h resulted in the statistical significantly highest (p ≤ 0.05) specific overall sugars productivity of (141 ± 1.4) × 10–4 g total sugars/g WF–LSWP/digestion step/h. Cultivation of C. tropicalis TISTR 5306 in digested and concentrated WF–LSWP extract at concentration level of 90 g/l during 0 – 12 h resulted in the following statistical significantly highest (p ≤ 0.05) kinetic parameters; specific growth rate (m) of 0.097 ± 0.001 h–1 and specific ethanol production rate (q<inf>P</inf>) of 0.221 ± 0.010 gP/gX/h. Dried biomass yield (Y<inf>X/S</inf>) and ethanol yield (Y<inf>P/S</inf>) based on utilized sugars of 90 g/l WF–LSWP extract at 0.180 ± 0.018 gX/gS and 0.411 ± 0.044 gP/gS, respectively, were statistical significantly highest (p ≤ 0.05) in comparison with those of 16 and 45 g/l WF–LSWP extracts.
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Effect of Media on Acetone-Butanol-Ethanol Fermentation by Isolated Clostridium spp.
(2017-01-01)
Buakhiaw, Budsaba
;
Sanguanchaipaiwong, Vorapat
Butanol is one of the important industrial chemicals and currently recognized as an alternative and renewable biofuel. It has been receiving an increased attention due to the energy crisis and the decrease of petroleum. Butanol can be obtained from several anaerobic microorganisms, which belong to genus Clostridium. The problem is the ability of Clostridium sp. to produce low concentration of butanol. Consequently, the screening and isolating for new Clostridium strains which can produce high amount of biobutanol has been required. Clostridium sp. strain G10 was isolated from marsh sediment in King Mongkut's Institute of Technology Ladkrabang (KMITL), Bangkok, Thailand. Its capability of butanol production was 5.89 g/L with a total acetone-butanol-ethanol (ABE) concentration of 8.48 g/L under anaerobic conditions. The medium composition significantly affected the performance of (ABE) production by strain G10. To investigate the effect of media on solvent production by isolated strain, four media, including RCM, T6, GYCC, and P2 medium (30 g/L glucose in each medium) were utilized to cultivate Clostridium sp. strain G10. The results revealed that, T6 medium could produce the highest biobutanol concentration (13.49 g/L) and ABE was 16.22 g/L (acetone 2.63 g/L and ethanol 0.10 g/L).
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PRETREATMENT AND HYDROLYSIS OF EUCALYPTUS FOR BIOBUTANOL PRODUCTION USING CLOSTRIDIUM SP. G10
(2025-01-01)
Rinthanapipat, Tunlaya
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Wongyou, Tarinee
;
Sanguanchaipaiwong, Vorapat
The primary objective of this study was to optimize the pretreatment and hydrolysis of eucalyptus for the cultivation of Clostridium sp. G10, a laboratory-isolated strain. Optimal alkali pretreatment conditions were determined to be 0.6 M NaOH at 121°C and 15 psi for 20 minutes, yielding a reducing sugar concentration of 11.91 g/L. Subsequent cellulose hydrolysis using ACCELERASE® 1500 (2000 endoglucanase U/g eucalyptus) for 24 hours further increased the reducing sugar concentration to 15.53 g/L. Eucalyptus hydrolysate was then utilized as a carbon source for Clostridium sp. G10 cultivation. In T6 medium supplemented with 50 g/L glucose, the maximum concentration of acetone-butanol-ethanol was 2.7 g/L. When T6 medium was supplemented with eucalyptus hydrolysate (50 g/L reducing sugar equivalent), the solvent concentration was found at 1.21 g/L. The results clearly indicate that the type of medium significantly impacts the production of acetone, butanol, and ethanol by Clostridium sp. G10. While eucalyptus hydrolysate can serve as a suitable carbon source, further optimization of hydrolysis conditions is necessary to achieve higher reducing sugar concentrations. Additionally, the removal of fermentation inhibitors from the hydrolysate is crucial for enhancing the efficiency of acetone-butanol-ethanol fermentation.
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Evaluation of cell disruption for partial isolation of intracellular pyruvate decarboxylase enzyme by silver nanoparticles method
(2015-07-01)
Tangtua, J.
;
Techapun, C.
;
Pratanaphon, R.
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Kuntiya, A.
;
Chaiyaso, T.
Candida tropicalis TISTR 5350 was used in the comparison of seven concentration levels of silver nanoparticles (0, 5, 10, 15, 20, 25, and 30 μg ml-1) for cell disruption methods. The optimized cell disruption strategy was selected based on the optimal protein yield and biological activity. The maximum volumetric and specifi c pyruvate decarboxylase (PDC, EC 4.1.1.1) activities (0.53±0.05 U ml-1 and 0.17±0.02 U mg-1 protein, respectively) were observed at 15 μg ml-1 silver nanoparticles. The silver nanoparticle concentration level of 15 μg ml-1 was investigated further by comparing the reaction mixtures at different time intervals of 0, 1, 2, 3, 4, 5, and 6 min. The result showed that the highest specifi c PDC activity of 0.39±0.01 U mg-1 protein was obtained from mixing for 3 min. This was not significantly different (P≤0.05) from other mixing time intervals.
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Pretreated and enzyme-hydrolyzed peanut shell for cultivation of clostridium sp. g10
(2021-01-01)
Sanguanchaipaiwong, V.
;
Yoddamnern, P.
;
Matngammnuang, W.
;
Leksawasdi, N.
Peanut shell (PS) is an agricultural residue and generated in large quantities at the peanut processing location. Normally, these lignocellulosic materials are utilized as feed or disposed of to landfill or burnt. In this research, PS was pretreated, enzymatically hydrolyzed, and utilized as a carbon source for Clostridium sp. G10 to produce butanol through acetone-butanol-ethanol (ABE) fermentation. PS was pretreated with H<inf>2</inf>SO<inf>4</inf> and NaOH (0- 1.0M). The highest reducing sugar concentration in the supernatant obtained from 0.4M H2SO4 was 23.1 g/L. Subsequently, the solid portion of pretreated PS was hydrolyzed at 50 °C for 24 h using commercial cellulose (ACCELLERASE™ 1500) with various treatment times duration and enzyme activity to solid ratios. The result suggested that enzyme activityof pretreated PS weight ratio of 1250 carboxymethyl cellulose (CMC) U:1 g yielded the maximum concentration of reducing sugars(12.74 g/L). Mixture of acidic pretreatment hydrolysate and enzyme hydrolysate contained41.2 g/L glucose concentration. Clostridium sp. G10 was then cultured in three conditions; T6 medium with 45 g/L glucose as a control, T6 medium prepared with 45 g/L reducing sugar equivalent of PS hydrolysate, and PS hydrolysate with 45 g/L reducing sugar equivalent at 37°C for 168 h. The implementation ofPS hydrolysate solely as a carbon source produced the butanol concentration of 10.8 g/L. This was not significantly different (p < 0.05) from using T6 medium with glucose (9.3 g/L). The highest butanol production of 15.2 g/Lwas yielded from T6 medium with PS hydrolysate. It could be concluded that hydrolyzed PS could be utilized as a carbon source for production of butanol and ethanol.
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Development of mathematical model for pyruvate decarboxylase deactivation kinetics by benzaldehyde with inorganic phosphate activation effect
(2018-05-01)
Khemacheewakul, Julaluk
;
Techapun, Charin
;
Kuntiya, Ampin
;
Sanguanchaipaiwong, Vorapat
;
Chaiyaso, Thanongsak
The effect of phosphate concentrations at 20, 250, 500, and 1,000 mM on phenylacetylcarbinol (PAC) production, pyruvate decarboxylase (PDC) deactivation kinetics, and combination of phosphate activation effect in a mathematical model were evaluated in a biotransformation system using whole cells of Candida tropicalis TISTR 5350. This is the first report of phosphate activation effect on pyruvate decarboxylase deactivation model. The highest PAC concentration (28.6 ± 2.3 mM), average instantaneous PAC formation rate (0.57 ± 0.01 mM/min), PAC yields (0.95 ± 0.08 on benzaldehyde and 0.71 ± 0.06 on pyruvate) were achieved in 1,000 mM phosphate buffer. PDC volumetric activity of 0.52 ± 0.07 U carboligase/ml at the reaction time of 180 min was obtained. The mathematical model describing deactivation kinetics of whole cells PDC by benzaldehyde with activation effect for phosphate buffer concentration level predicted individual experimental data for all four levels of phosphate buffer relatively well with corresponding residual sum of square (RSS), mean square (MS), and correlation coefficient (R2) range of 213-1,100, 5.32-27.5, and 0.96-0.99. The activation effect of 1,000 mM phosphate buffer was evident with an average enzyme activation rate constant due to buffering species concentration level (K<inf>a</inf>) of 1.34 × 10-2 % min-1 which was higher than 20 mM phosphate buffer (1.48 × 10-6 % min-1) by more than 9,050 times.
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Partial purification and comparison of precipitation techniques of pyruvate decarboxylase enzyme
(2017-01-01)
Tangtua, Julaluk
;
Techapun, Charin
;
Pratanaphon, Ronachai
;
Kuntiya, Ampin
;
Sanguanchaipaiwong, Vorapat
The intracellular pyruvate decarboxylase enzyme (PDC, EC 4.1.1.1) extract from Candida tropicalis TISTR 5350 was compared by two different purification methods using ammonium sulphate and acetone precipitation. The total volumetric PDC activity and percentage recovery (yield) of precipitated PDC based on 50% (v/v) cold acetone were significantly higher (1.13 ± 0.02 U/ml and 98.27 ± 2.98 %, respectively) than any other concentration levels of acetone used. Furthermore, all concentration levels of cold acetone also yielded a much higher specific PDC activity than the precipitate obtained using the 40 to 60% (w/v) ammonium sulphate saturation (0.75 ± 0.08 U/mg protein). The precipitated enzyme in buffer solutions from the 50% (v/v) acetone was subsequently freeze dried. Freeze drying of the precipitated PDC by cold acetone resulted in the specific PDC activity of 1.57 ± 0.02 U/mg protein and differed statistically (p ≤ 0.05) from the crude enzyme extract (control).

Sanguanchaipaiwong, Vorapat

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