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Molecular genetic diversity of Bambara groundnut (Vigna subterranea L. Verdc.) revealed by rapd and ISSR marker analysis
Author(s)
Rungnoi, O.
Suwanprasert, J.
Somta, P.
Srinives, P.
Date Issued
December 13, 2012
Type
Article
Abstract
A set of 363 Bambara groundnut (Vigna subterranea L. Verdc.) accessions from five geographical regions and unknown origin was assessed with 65 loci generated from inter simple sequence repeat (ISSR) and random amplified polymorphic DNA (RAPD) markers to provide more information on genetic diversity and origin. Higher percentage of polymorphic DNA bands, gene diversity and Shannon's diversity index were found in accessions from Cameroon/Nigeria and West Africa regions but not much different from those found in accessions from West Africa. Genetic distance revealed close genetic relationships between accessions from Cameroon/Nigeria and West Africa regions. Hierarchical cluster analysis revealed that Thai Bambara groundnuts are clustered with both West and East Africa regions. Principal coordinate analysis demonstrated that accessions from Cameroon/Nigeria and West Africa were separated into two groups, where accessions from the two regions were mingled with each others. Although most accessions from East Africa were grouped with a cluster of Cameroon/Nigeria and West Africa accessions. Population structure analysis grouped the germplasm into two subpopulations where most accessions were in one subpopulation and 11 accessions were in the other subpopulation. Altogether, the results support the views that West Africa (including Cameroon and Nigeria) is the center of diversity of Bambara groundnut. The crop was introduced from West to East Africa, from where the Thai accessions were introduced.
Citation
Sabrao Journal of Breeding and Genetics, 44(1), 87-101, 2012