Loading...
Streptomyces chumphonensis sp. nov., isolated from marine sediments
Author(s)
Phongsopitanun, Wongsakorn
Suwanborirux, Khanit
Kudo, Takuji
Ohkuma, Moriya
Tanasupawat, Somboon
Date Issued
January 1, 2014
Type
Article
Abstract
Two actinomycete strains, KK1-2T and CPB4-7, were isolated from marine sediments collected in Chumphon province, Thailand. Chumphon province, Thailand. Their taxonomic positions were determined using a polyphasic approach. The morphological, cultural and chemotaxonomic characteristics of these isolates were consistent with the classification of the strains as representing a member of the genus Streptomyces. They contained LL-diaminopimelic acid in their cell wall peptidoglycan; the whole-cell sugars were ribose and glucose. The predominant menaquinones were MK9-(H6) and MK9-(H8). The major polar lipids were phosphatidylethanolamine, phosphatidylinositol, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol mannosides. The predominant cellular fatty acids were anteiso-C15: 0, iso-C16: 0 and iso-C15: 0. On the basis of 16S rRNA gene sequence similarity studies, these isolates were determined to be closely related to Streptomyces xinghaiensis JCM 16958T (98.2 %), Streptomyces rimosus subsp. paromomycinus JCM 4541T (98.1 %), Streptomyces sclerotialus JCM 4828T (98.1 %) and Streptomyces flocculus JCM 4476T (98.0 %). The G+C contents of the genomic DNA of strains KK1-2T and CPB4-7 were 73.3 and 74.2 mol%, respectively. They could be clearly distinguished from the related type strains by a low DNA-DNA relatedness and phenotypic differences. On the basis of these results, these strains represent a novel species of the genus Streptomyces, for which the name Streptomyces chumphonensis sp. nov. (type strain KK1-2T = JCM 18522T = TISTR 2106T = PCU 330T) is proposed. © 2014 IUMS.
Citation
International Journal of Systematic and Evolutionary Microbiology, 64(PART 8), 2605-2610, 2014