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Planobispora takensis sp. nov., isolated from soil
Author(s)
Ngaemthao, Wipaporn
Suriyachadkun, Chanwit
Chunhametha, Suwanee
Niemhom, Nantawan
Sanglier, Jean Jacques
Date Issued
January 1, 2014
Type
Article
Abstract
A novel Gram-stain-positive, filamentous bacterial strain (A-T 7458T) was isolated from soil collected from hill evergreen forest in Thailand. The strain developed cylindrical sporangia containing a longitudinal pair of motile spores, on short ramifications of aerial mycelia. From a taxonomic study using a polyphasic approach, strain A-T 7458Thad typical characteristics of members of the genus Planobispora. 16S rRNA gene sequence analysis indicated that the isolate was closely related to Planobispora siamensis A-T 4600T(98.5 %), Planobispora rosea JCM 3166T(97.6 %) and Planobispora longispora NBRC 13918T(97.6 %). The DNA-DNA relatedness values, which differentiated the novel strain from the closest species, were significantly below 70 %. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained ribose, madurose, mannose and glucose. The predominant menaquinone was MK-9(H2). The diagnostic phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylinositol, phosphatidylinositol-mannoside and aminophosphoglycolipid. The predominant cellular fatty acids were unsaturated C17:1 and C18:1, and saturated C16:0 and C17:0. Following an evaluation of phenotypic, chemotaxonomic and genotypic characteristics, the novel isolate is proposed to represent a novel species of the genus Planobispora, to be named Planobispora takensis sp. nov. The type strain is A-T 7458T(=BCC 48396T=NBRC 109077T). © 2014 IUMS.
Citation
International Journal of Systematic and Evolutionary Microbiology, 64(PART 4), 1180-1185, 2014