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Phytoalexin production of lettuce (Lactuca sativa L.) grown in hydroponics and its in vitro inhibitory effect on plant pathogenic fungi
Author(s)
Date Issued
September 1, 2017
Type
Article
Abstract
A series of experiments were conducted to investigate phytoalexin production from five varieties of lettuce in hydroponics using abiotic (2.5, 5% CuSO4; 0.5, 1% AgNO3) and biotic elicitors (non-pathogenic Pythium sp.) at different plant ages. It showed that phytoalexin was successfully induced in tested lettuce grown in hydroponics after elicitation with abiotic elicitors throughout the trial. Phytoalexin showed yellow fluorescent spot under 365 nm UV light with Rf 0.45-0.48 and clear inhibition zone where Aspergillus niger failed to develop on TLC plate. For biotic elicitors, no yellow fluorescent spot on TLC plate was observed from tested lettuce varieties however inhibition zone at Rf 0.9 was detected at 8-9 weeks from red oak, green oak, and red coral. Moreover, crude extract of lettuce elicited with abiotic elicitors possessed in vitro antifungal activity against C. gloeosporioides, C. lunata, F. oxysporum, and P. aphanidermatum probably due to phytoalexin (lettucenin A) in the extract.
Citation
Songklanakarin Journal of Science and Technology, 39(5), 633-640, 2017