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Uses of copra waste and wheat bran for cellulase production by trichoderma reesei in solid state fermentation
Author(s)
Kittanan, Thammaratchai
Nareerat, Lexpet
Sawwanit, Kaisaard
Teerin, Chysirichote
Date Issued
January 18, 2018
Type
Conference Paper
Abstract
The potential use of copra waste from coconut pressing process for cellulase production by solid-state fermentation (SSF) was studied in this research. Trichoderma reesei TISTR3080 was cultivated at 30°C for 7 days on the copra waste (CW) with and without supplementary of wheat bran (WB) as a nitrogen source. The results showed that adding WB increased both the fungal growth and productions of cellulase including activities of total cellulase, CMCase and Β-glucosidase. The fungal growth which was indirectly measured by glucosamine measurement increased from 0.33 d-1 in the CW without WB to 1.1 d-1 in the CW added WB at the ratio of 3:3. Moreover, adding WB to CW at the ratio of 3:3 accelerated the activity of Β-glucosidase from 12.6 to 16.8 U/ g dry substrate at 1 d. However, supplementing CW with WB at the ratio of 3:2 enhanced the total cellulase activity and CMCase activity from 0.31 to 5.23 FPU/ g dry substrate at 2 d and 0.42 to 5.18 U/ g dry substrate at 6 d, respectively.
Citation
ACM International Conference Proceeding Series, 56-59, 2018