Ultrasonication on Collagen Yield, Physiochemical and Structural Properties from Seabass (Lates Calcarifer) Scales as Affected by Pretreatment and Extraction Conditions

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dc.contributor.authorBavisetty, Sri Charan Bindu
dc.contributor.authorKarnjanapratum, Supatra
dc.contributor.authorDave, Jaydeep
dc.contributor.authorPurba, Daniel Tua
dc.contributor.authorKudre, Tanaji
dc.contributor.authorMaser, Wahyu Haryati
dc.contributor.authorMaiyah, Nur
dc.contributor.authorKingwascharapong, Passakorn
dc.contributor.authorAli, Ali Muhammed Moula
dc.date.accessioned2026-08-06T10:44:33Z
dc.date.available2026-08-06T10:44:33Z
dc.date.issued2024-01-01
dc.description.abstractThis research explored the use of ultrasound as a pretreatment and extraction assisted process for extraction of collagen from seabass scales. Both acid-soluble (AC) and pepsin-soluble collagens (PC) were extracted with ultrasonication at different extraction times (24 and 48 h). Ultrasound pretreatment (PU) and ultrasound-assisted extraction (UE) notably improved AC extraction yields over 48 hours, registering 2.12% and 4.62%, respectively. The highest yield for PC, 21.85%, was achieved with 48 hours of ultrasound assisted extraction. Protein analysis verified the extracted collagens as type I, indicated by the presence of specific α and β chains. Further, FTIR spectra confirmed collagen's presence in all samples through distinct amide band peaks. Interestingly, ultrasound processes marginally reduced AC's thermal stability while boosting PC's thermal stability, especially when combined with pepsin treatment. Moreover, CD spectroscopy showed the preserved native structure of collagen across all samples. The ultrasound method increased collagen extraction yields and retained its structural integrity. These outcomes are pivotal for advancing collagen extraction methods in the biomedical and food sectors.
dc.identifier.citationNatural and Life Sciences Communications, 23(1), 2024
dc.identifier.doi10.12982/NLSC.2024.003
dc.identifier.issn28220838
dc.identifier.other2-s2.0-85195567029
dc.identifier.urihttps://dspace.kmitl.ac.th/handle/123456789/15228
dc.sourceNatural and Life Sciences Communications
dc.subjectCollagen
dc.subjectMolecular characterization
dc.subjectPhysicochemical properties
dc.subjectSeabass scales
dc.subjectUltrasonication
dc.titleUltrasonication on Collagen Yield, Physiochemical and Structural Properties from Seabass (Lates Calcarifer) Scales as Affected by Pretreatment and Extraction Conditions
dc.typeArticle

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