Multiplexed Surface-Enhanced Raman Mapping via DIY Spectrometer for High-Specificity Molecular Detection

Abstract

In this research, we aim to build a low-cost Do-It-Yourself(DIY) Raman spectrometer system. Due to the previous limitation, the surface-enhanced Raman spectroscopy(SERS) multiplexing for enhance the Raman fingerprint of the benchmark substrate. The previous research was done at a single point, then 3×2 multiplex mapping only. This study enables the 4×4 multiplex mapping method using SERS substrates. Expanding beyond previous work on single-point detection, this study demonstrates a 4×4 multiplex mapping method using SERS substrates (S440) nanoparticle-based substrate. This allows a deeper range of the DIY Raman to get multiple Raman fingerprints from the same substrate and leads to the future artificial intelligence(AI) identification of the substrate complexity and the quantitative analysis. The system is based on a 785nm fiber laser and a custom optics setup.