Antibacterial activity of marine microalgae extracts against pathogen of Siamese fighting fish (Betta splendens)

Abstract

Background: Marine microalgae extracts are a promising alternative to commercial drugs because of their antimicrobial properties. Aim: . Methods: Crude extracts from microalgae were obtained via maceration using ethanol and hexane as solvents. Antibacterial activities were initially screened using the agar well diffusion method, whereas antimycobacterial activity was assessed using the microplate 7H11 agar proportion method. Subsequently, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the crude extracts were determined. Gas chromatography-mass spectrometry (GC-MS) was used for phytochemical composition analysis to identify significant antimicrobial compounds in the extracts. Results: revealed the presence of several bioactive compounds. The major components identified include Phthalate, Tetradecanoic acid, Hexadecanoic acid, and Phytol. Conclusion: in betta fish. These results highlight the potential of marine microalgae as natural, sustainable alternatives for disease control in aquaculture.