Optimization for Callus Induction and Plant Regeneration from Mature Seeds of Thai Rice Variety: Nam Roo (Oryza sativa L.)

Abstract

The aim of this study was to develop a productive protocol for optimum callus induction and regeneration of Thai rice variety: Nam Roo (Oryza sativa L.).Callus was induced on either MS (Murashige and Skoog) or NB (Nitsch and Nitsch) media supplemented with various concentrations of 2,4-D (2,4-dichlorophenoxya cetic acid), 0.5 mg/l NAA (α-naphthaleneacetic acid), 1 g/l L-proline, 30 g/l sucrose and 2.6 g/l phytagel.Callus from Nam Roo seed gave its maximum mean size (391.49mm 3 ) and mean weight (0.3412 g.) on NB medium supplemented with 1 mg/l 2, 4-D for 4 weeks.For plant regeneration the callus was cultured on either MS or NB media containing with different concentrations of BAP (6-benzylaminopurine) and phytagel, 0.5 mg/l NAA and 30 g/l sucrose.The highest regeneration frequency (%) was observed from callus grown on MS medium composed of 2 mg/l BAP in 5.2 g/l phytagel.It took 6 weeks for the callus to regenerate into a complete plant.Finally, the plantlets were transferred into the plastic pot.